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| Web URL(s): | http://www.nrcresearchpress.com/doi/pdf/10.1139/b89-445
Last checked: 09/29/2015
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Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Reaney, M. J. T.;
Gusta, L. V.;
Abrams, S. R.;
Robertson, A. J. |
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| Author Affiliation: | University of Saskatchewan, University of Saskatchewan, Plant Biotechnology Institute, and University of Saskatchewan, respectively. |
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| Title: | The Effects of Abscisic Acid, Kinetin, and Gibberellin on Freezing Tolerance in Smooth Bromegrass (Bromus inermis) cell suspensions. |
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| Source: | Canadian Journal of Botany. Vol. 67, No. 12, December 1989, p. 3640-3646. |
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| Publishing Information: | Vancouver, British Columbia: The National Research Council of Canada. |
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| Related Web URL: | http://www.nrcresearchpress.com/doi/abs/10.1139/b89-445
Last checked: 09/29/2015
Notes: English abstract only |
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| Keywords: | TIC Keywords: Kinetin; Gibberellic acid; Gibberellins; Cold resistance; Bromus inermis; Abscisic acid; Growth
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| Abstract/Contents: | "The effects of kinetin and gibberellic acids (GA3, GA4, GA7, GA9, and a mixture of GA4,7,9,) on cold hardening, dehardening, and growth of smooth bromegrass (Bromus inermis Leyss. cv. Manchar) suspension cultures treated with abscisic acid (ABA) were determined. Bromegrass cells treated with 75 muM racemic ABA for 7 days at 25 degrees C tolerated -37 degrees C, whereas cells treated with both racemic ABA (75muM) and a mixture of GA4, GA7, and GA9, (total gibberellic acid concentration 100 muM) were similar in hardiness to the controls (LT50, -10 degrees C). GA4,7,9 at concentrations greater than 10 muM inhibited the growth of cells. Although 400 muM GA4,7,9 was lethal to cells, 75 muM ABA overcame the lethal effect but did not overcome growth inhibition. The twofold reduction in cell water content due to 75 muM ABA treatment for 7 days was partially overcome by GA4,7,9 at concentrations greater than 400 muM. GA4, GA7, and GA9, were equally effective at limiting growth and inhibiting freezing tolerance induced by ABA, whereas GA3 had little effect on cold hardiness, growth, and water content. During the first 4 days, kinetin at concentrations greater than 100 muM inhibited growth of both control cells and cells treated with ABA. Kinetin (>100 muM) also inhibited freezing tolerance induced by abscisic acid after 4 days, but had no effect after 8 days. Bromegrass cells treated with 75 muM ABA for 7 days were after transfer to fresh medium minus ABA after 12 days at 10 degrees C. GA4,7,9 (40 muM) had no effect on the rate of dehardening, whereas kinetin increased the rate of dehardening." |
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| Language: | English |
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| References: | 28 |
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| Note: | Abstract also appears in French
Tables
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Reaney, M. J. T., L. V. Gusta, S. R. Abrams, and A. J. Robertson. 1989. The Effects of Abscisic Acid, Kinetin, and Gibberellin on Freezing Tolerance in Smooth Bromegrass (Bromus inermis) cell suspensions.. Can. J. Bot. 67(12):p. 3640-3646. |
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| Web URL(s):
http://www.nrcresearchpress.com/doi/pdf/10.1139/b89-445
Last checked: 09/29/2015
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: QK 1 .C3 |
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