| |
| DOI: | 10.2135/cropsci2010.04.0215 |
|---|
| Web URL(s): | https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2010.04.0215
Last checked: 03/24/2024
Access conditions: Item is within a limited-access website
https://acsess.onlinelibrary.wiley.com/doi/epdf/10.2135/cropsci2010.04.0215
Last checked: 03/24/2024
Requires: PDF Reader
Access conditions: Item is within a limited-access website
http://www.umass.edu/turfpathology/documents/Mite_manuscript_2011.pdf
Last checked: 03/16/2011
Requires: PDF Reader |
|---|
| Publication Type:
| Refereed |
|---|
| Author(s): | Amundsen, Keenan;
Rotter, David;
Li, Huaijun Michael;
Messing, Joachim;
Jung, Geunhwa;
Belanger, Faith;
Warnke, Scott |
|---|
| Author Affiliation: | Amundsen and Warnke: USDA-ARS, Floral and Nursery Plants Research Unit, Beltsville, MD; Rotter: Univ. of Texas Southwestern Medical Center, Dallas, TX; Li and Belanger: Plant Biology and Pathology, Rutgers Univ., New Brunswick; Messing: Waksman Institute of Microbiology, Rutgers Univ., Piscataway, NJ; Jung: Plant, Soil and Insect Sciences, Univ. of Massachusetts, Amherst, MA |
|---|
| Title: | Miniature inverted-repeat transposable element identification and genetic marker development in Agrostis |
|---|
| Section: | Turfgrass science
Other records with the "Turfgrass science" Section
|
|---|
| Source: | Crop Science. Vol. 51, No. 2, March 2011, p. 854-861. |
|---|
| Publishing Information: | Madison, WI: Crop Science Society of America |
|---|
| # of Pages: | 8 |
|---|
| Keywords: | TIC Keywords: Agrostis stolonifera; Alleles; DNA amplification; Genetic markers; Genetic variability; Identification; Polymerase chain reaction; Software
|
|---|
| Abstract/Contents: | "Creeping bentgrass (Agrostis stolonifera L.) is an important species to the turfgrass industry because of its adaptation for use in high quality turf stands such as golf course putting greens, tees, and fairways. Creeping bentgrass is a highly outcrossing allotetraploid, making genetic marker development difficult. Genetic markers anchored to miniature inverted-repeat transposable elements (MITEs) were developed in Agrostis that could be used in genetic linkage mapping, quantitative trait loci studies, or diversity analyses. The FindMITE software program identified 495 candidate MITEs from 16,122 Agrostis DNA sequences. There was evidence of transposition in 79 of the candidate MITEs based on MITE insertional polymorphisms. Genetic markers were developed by MITE-display, a modified amplified fragment length polymorphism technique that anchors amplified fragments to MITEs. Four MITE-display primer combinations were tested on a creeping bentgrass experimental mapping population and 139 polymorphic markers were developed with a polymorphic information content of 0.33. Twenty-eight of the polymorphic genetic markers segregated normally. MITE-display genetic markers are a new class of genetic markers for studies of the Agrostis genome. These genetic markers target transposable elements and provide an easy method of identifying allelic variation between Agrostis accessions that may be used for diversity studies or genetic linkage map development." |
|---|
| Language: | English |
|---|
| References: | 56 |
|---|
| Note: | Pictures, b/w
Figures
Tables |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Amundsen, K., D. Rotter, H. M. Li, J. Messing, G. Jung, F. Belanger, et al. 2011. Miniature inverted-repeat transposable element identification and genetic marker development in Agrostis. Crop Sci. 51(2):p. 854-861. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=177753 |
| If there are problems with this record, send us feedback about record 177753. |
| Choices for finding the above item: |
|---|
| DOI: 10.2135/cropsci2010.04.0215 |
| Web URL(s):
https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2010.04.0215
Last checked: 03/24/2024
Access conditions: Item is within a limited-access website
https://acsess.onlinelibrary.wiley.com/doi/epdf/10.2135/cropsci2010.04.0215
Last checked: 03/24/2024
Requires: PDF Reader
Access conditions: Item is within a limited-access website
http://www.umass.edu/turfpathology/documents/Mite_manuscript_2011.pdf
Last checked: 03/16/2011
Requires: PDF Reader |
| 
MSU catalog number: b2211522a |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
