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| DOI: | 10.1007/s11032-010-9475-4 |
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| Web URL(s): | https://link.springer.com/article/10.1007%2Fs11032-010-9475-4
Last checked: 10/05/2017 |
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| Publication Type:
| Refereed |
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| Author(s): | Chandra-Shekara, A. C.;
Pegadaraju, Venkatramana;
Thompson, Michael;
Vellekson, Donn;
Schultz, Quentin |
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| Author Affiliation: | Chandra-Shekara, Pegadaraju, Thompson and Schultz: BioDiagnostics Inc, River Falls, WI; Vellekson: Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN |
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| Title: | A novel DNA-based diagnostic test for the detection of annual and intermediate ryegrass contamination in perennial ryegrass |
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| Source: | Molecular Breeding. Vol. 28, No. 2, August 2011, p. 217-225. |
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| Publishing Information: | The Netherlands: Kluwer Academic Publishers |
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| # of Pages: | 9 |
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| Related Web URL: | http://www.springerlink.com/content/l818795k86478660/
Last checked: 05/25/2011
Notes: Abstract only |
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| Keywords: | TIC Keywords: Competition; Cost efficiency; Cultivar identification; DNA amplification; Genetic markers; Lolium multiflorum; Lolium perenne; Lolium x hybridum; Polymerase chain reaction; Quantitative trait loci; Seed industry; Vernalization
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| Abstract/Contents: | "Perennial ryegrass (Lolium perenne L.) is a preferred choice for the turf grass industry due to its ability to provide a durable turf cover. Genetic or physical contamination of annual (L. multiflorum Lam.) or intermediate (L. hybridum) ryegrass species in perennial ryegrass is one of the major problems affecting the grass seed industry. At present, seedling root fluorescence (SRF), a biochemical marker, is used for the detection of annual ryegrass contamination. Due to the unreliability of the SRF test, the seed industry is seeking an alternative, more reliable and accurate detection method. Currently, there are no DNA tests available in ryegrass for detecting contamination with annual and intermediate ryegrass types. We developed a novel quantitative polymerase chain reaction (Q-PCR)-based DNA test for the detection of annual and/or intermediate ryegrass types in perennial ryegrass. This DNA test was designed using an insertion/deletion (InDel) site in the LpVRN2 2 (Vernalization 2) gene, which is one of the several genes controlling vernalization in ryegrass. The new DNA test is more reliable, accurate and cost-effective in detecting contamination, with a high sensitivity of 0.04% in a sample size of 5,000 seeds. Use of larger sample sizes (12.5-fold higher compared to SRF test) provided additional accuracy in detecting the level of contamination. The method has produced consistent results in 68 perennial, 26 annual and 14 intermediate ryegrass lines." |
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| Language: | English |
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| References: | 19 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Chandra-Shekara, A. C., V. Pegadaraju, M. Thompson, D. Vellekson, and Q. Schultz. 2011. A novel DNA-based diagnostic test for the detection of annual and intermediate ryegrass contamination in perennial ryegrass. Molecular Breeding. 28(2):p. 217-225. |
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| DOI: 10.1007/s11032-010-9475-4 |
| Web URL(s):
https://link.springer.com/article/10.1007%2Fs11032-010-9475-4
Last checked: 10/05/2017 |
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