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| DOI: | 10.2135/cropsci2010.08.0478 |
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| Web URL(s): | https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2010.08.0478
Last checked: 03/24/2024
Access conditions: Item is within a limited-access website
https://acsess.onlinelibrary.wiley.com/doi/epdf/10.2135/cropsci2010.08.0478
Last checked: 03/24/2024
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Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Kamps, Terry L.;
Williams, Neil R.;
Ortega, Victor M.;
Chamusco, Karen C.;
Harris-Shultz, Karen;
Scully, Brian T.;
Chase, Christine D. |
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| Author Affiliation: | Kamps, Williams, Ortega, Chamusco and Chase: Horticultural Sciences Dep., University of Florida, Gainesville, FL; Harris-Shultz and Scully: USDA-ARS, Coastal Plain Experiment Station, Tifton, GA |
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| Title: | DNA polymorphisms at bermudagrass microsatellite loci and their use in genotype fingerprinting |
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| Section: | Genomics, molecular genetics and biotechnology
Other records with the "Genomics, molecular genetics and biotechnology" Section
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| Source: | Crop Science. Vol. 51, No. 3, May 2011, p. 1122-1131. |
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| Publishing Information: | Madison, WI: Crop Science Society of America |
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| # of Pages: | 10 |
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| Keywords: | TIC Keywords: Amplification fragment length polymorphisms; Cultivar identification; Cynodon dactylon; Cynodon transvaalensis; DNA amplification; Genotyping; Microsatellites; Simple sequence repeats
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| Abstract/Contents: | "The turf-type bermudagrasses include diploid Cynodon transvaalensis Burtt Davy, tetraploid C. Dactylon (L.) Pers., and sterile triploid hybrids produced by crosses of these species. The objective of this study was to develop a set of microsatellite markers that could distinguish among commercially important turf-type cultivars. A genomic library enriched for the [CA/GT]n repeat motif was constructed from DNA of the 'Tifway' hybrid and sequenced to identify microsatellite regions. Twenty-five microsatellite-flanking primer sets were developed and used to genotype two plant introductions and 12 turf-type cultivars. These primer sets produced an average of 10 amplicons across the 14 DNA templates. Sequences of selected amplicons revealed polymorphism resulting from expansion and/or contraction of the microsatellite and from indel mutations in the microsatellite flanking regions. As few as two primer sets were sufficient to differentiate all unrelated introduction lines and cultivars. The primer sets failed to distinguish among closely related cultivars developed by selection of natural variants, but one primer set uniquely distinguished the cultivar TifEagle from its irradiated parent cultivar. These genomic microsatellites were not derived from gene coding sequences and will supplement the existing expressed sequence tag (EST)-based bermudagrass microsatellites. They will be most useful for evaluating the genetic diversity of Cynodon accessions and distinguishing among cultivars that exploit this diversity." |
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| Language: | English |
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| References: | 72 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Kamps, T. L., N. R. Williams, V. M. Ortega, K. C. Chamusco, K. Harris-Shultz, B. T. Scully, et al. 2011. DNA polymorphisms at bermudagrass microsatellite loci and their use in genotype fingerprinting. Crop Sci. 51(3):p. 1122-1131. |
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| DOI: 10.2135/cropsci2010.08.0478 |
| Web URL(s):
https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2010.08.0478
Last checked: 03/24/2024
Access conditions: Item is within a limited-access website
https://acsess.onlinelibrary.wiley.com/doi/epdf/10.2135/cropsci2010.08.0478
Last checked: 03/24/2024
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2211522a |
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