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DOI: | 10.2135/cropsci2010.09.0512 |
Web URL(s): | https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2010.09.0512 Last checked: 03/24/2024 Access conditions: Item is within a limited-access website https://acsess.onlinelibrary.wiley.com/doi/epdf/10.2135/cropsci2010.09.0512 Last checked: 03/24/2024 Requires: PDF Reader Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Amundsen, Keenan;
Warnke, Scott |
Author Affiliation: | Floral and Nursery Plants Research Unit, USDA-ARS, Beltsville, MD |
Title: | Species relationships in the genus Agrostis based on flow cytometry and MITE-display molecular markers |
Section: | Turfgrass science Other records with the "Turfgrass science" Section
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Source: | Crop Science. Vol. 51, No. 3, May 2011, p. 1224-1231. |
Publishing Information: | Madison, WI: Crop Science Society of America |
# of Pages: | 8 |
Keywords: | TIC Keywords: Agrostis; Apera; Genetic diversity; Germplasm; Interspecific hybridization; Laser flow cytometry; Molecular markers; Ploidy; Polypogon monspeliensis
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Abstract/Contents: | "It is estimated that there are between 150 and 200 species of Agrostis, and interspecific hybridization is a proven method for improving cultivated Agrostis species. The pool of publicly available Agrostis germplasm, available through the National Plant Germplasm System, represents 17% of the total number of Agrostis species. The total amount of genetic diversity of this germplasm should be assessed to determine if there are novel sources of stress resistance. Agrostis species have a base haploid chromosome number of seven and can range in ploidy from diploid (2n = 2x = 14) to dodecaploid (2n = 12x = 84). In the present study, the DNA content of 305 Agrostis accessions was measured by flow cytometry and used to predict ploidy. Ploidy assignment was difficult because the DNA content measures did not delineate the accessions into clear genome size groupings. Genetic diversity among a subset of those Agrostis accessions (75), along with four Polypogon and two Apera accessions, was also studied. Genetic markers anchored to miniature inverted-repeat transposable elements (1309) were used in Structure 2.3.3 and unweighted pair-grouped method analyses. Eight distinct clusters of accessions were observed, including two distinct groups of diploid germplasm. Little genetic variation was evident among the cultivated A. Stolonifera accessions based on the Structure analysis, suggesting the early formation of a genetic bottleneck among cultivated germplasm." |
Language: | English |
References: | 25 |
Note: | Figures Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Amundsen, K., and S. Warnke. 2011. Species relationships in the genus Agrostis based on flow cytometry and MITE-display molecular markers. Crop Sci. 51(3):p. 1224-1231. |
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| DOI: 10.2135/cropsci2010.09.0512 |
| Web URL(s): https://acsess.onlinelibrary.wiley.com/doi/10.2135/cropsci2010.09.0512 Last checked: 03/24/2024 Access conditions: Item is within a limited-access website https://acsess.onlinelibrary.wiley.com/doi/epdf/10.2135/cropsci2010.09.0512 Last checked: 03/24/2024 Requires: PDF Reader Access conditions: Item is within a limited-access website |
| MSU catalog number: b2211522a |
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