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| Web URL(s): | https://dl.sciencesocieties.org/publications/cs/pdfs/30/4/CS0300040947
Last checked: 12/09/2016
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Oprisko, Marianne J.;
Green, Robert L.;
Beard, James B.;
Gates, Charles E. |
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| Author Affiliation: | Texas A&M University, College Station |
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| Title: | Vital staining of root hairs in 12 Warm-season perennial grasses |
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| Source: | Crop Science. Vol. 30, No. 4, July/August 1990, p. 947-950. |
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| Publishing Information: | Madison, WI: Crop Science Society of America |
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| Keywords: | TIC Keywords: Root hairs; Drought resistance; Identification; Dyes
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| Abstract/Contents: | "Root hairs have been shown to absorb water and nutrients; thus, it may be important to characterize their quantity, size, and viability when studying components of drought resistance. The first objective was to find the most effective and precise vital stain for root hairs of warm-season perennial grasses. Sprigs of 12 genotypes representing six species were grown in sand culture in the greenhouse. Vital stain effectiveness in 0.05 M PO4 buffer (pH 7.0) was established by the percentage of genotypes in which vitality could be determined using the live/dead color difference. The five vital stains, with their percentage effectiveness over the 12 genotypes and their average absolute color difference values (none=1, greatest=4), were: 0.5 g/L Evan's blue, 100%, 3.3; 0.1 g/L methylene blue, 67%, 1.9; 1 g/L Congo red, 58%, 1.8; 0.1 g/L phenosafranin, 50%, 1.7; and 0.1 g/L neutral red, 25%, 1.4 Evan's blue was the best stain, in terms of greatest precision and least variability." |
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| Language: | English |
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| References: | 20 |
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| Note: | ERRATA: Presented in Crop Science 30:6 on p.1379. A sentence was scrambled on page 948, column 1.
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Oprisko, M. J., R. L. Green, J. B. Beard, and C. E. Gates. 1990. Vital staining of root hairs in 12 Warm-season perennial grasses. Crop Sci. 30(4):p. 947-950. |
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| Web URL(s):
https://dl.sciencesocieties.org/publications/cs/pdfs/30/4/CS0300040947
Last checked: 12/09/2016
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: SB 183 .C7 |
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