Full TGIF Record # 185427
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Author(s):Griffith, S. M.; Banowetz, G. M.
Author Affiliation:USDA-ARS, Natonal Forage Seed Production Research Center, Corvallis, Oregon
Title:Differentiation of Lolium perenne L. and L. multiflorum Lam. seed by two esterase isoforms
Source:Seed Science and Technology. Vol. 20, No. 3, 1992, p. 343-348.
# of Pages:6
Publishing Information:Zurich: International Seed Testing Association
Abstract/Contents:"When Lolium perenne L. (perennial ryegrass) seed is contaminated by L. multiflorum Lam. (Italian ryegrass), it cannot be marketed as certified seed. It would be useful if a reliable genetic marker could be found that would distinguish between L. perenne and L. multiflorum seed. One potential marker may be an L. multiflorum specific esterase, Est-1. The objectives of this research were: 1) to examine the seeds of 18 L. multiflorum and 74 L. perenne to determine if Est-1 activity is unique to L. multiflorum; and 2) to determine if Est-1 activity exists in seeds of other Lolium species. Analyses were performed using both native polyacrylamide gel electrophoresis (native-PAGE) and isoelectric focusing electrophoresis (IEF-PAGE). Following electrophoresis, gels were stained for esterase activity. Results of this investigation confirmed earlier reports showing the existence of a unique L. multiflorum seed esterase isoenzyme, not present in L. perenne. Through high resolution native-PAGE, a total of two Est-1 esterase isoforms (Est-1a and Est-1b) were identified. These forms were present together in seeds of all L. multiflorum cultivars examined. The pI of both Est-1 isoforms was 5.15. An esterase of 5.15 pI was also found in L. remotum Schrank. seed. Est-1 activity was not detected in L. canariense Steud., L. persicum Boiss., L. temulentum L., L. rigidum Gaudin., and L. subulatum Vis. It appears that both isoforms could be used as markers to distinguish between L. perenne and L. multiflorum seed."
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Griffith, S. M., and G. M. Banowetz. 1992. Differentiation of Lolium perenne L. and L. multiflorum Lam. seed by two esterase isoforms. Seed Sci. Technol. 20(3):p. 343-348.
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