Full TGIF Record # 185428
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Author(s):Greneche, M.; Lallemand, J.; Michaud, O.
Author Affiliation:Institut National de la Recherche Agronomique, Groupe d'Etude et de Contr√īle des Vari√©t√©s et des Semences, Surg√©res, France
Title:Comparison of different enzyme loci as a means of distinguishing ryegrass varieties by electrophoresis
Source:Seed Science and Technology. Vol. 19, No. 1, 1991, p. 147-158.
# of Pages:12
Publishing Information:Zurich: International Seed Testing Association
Abstract/Contents:"Six enzymes have been studied as a means of distinguishing between ryegrass varieties: acid phosphatase (ACP), isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH), phosphoglucoisomerase (PGI), shikimate dehydrogenase (ShDH), superoxide dismutase (SOD). The results found were in good agreement with the results obtained at different locations by different authors. At the ACP locus, three additional alleles, not previously reported, were found and a good distinctness power was obtained. For the IDH locus, five alleles were recorded allowing the distinction of varieties especially of the species Lolium multiflorum. The SOD locus, with only 2 alleles, and the ShDH locus, which showed little genetic variation, cultivars being largely homozygous, were the least useful enzymes for distinguishing ryegrass varieties. Further study of MDH will be necessary before it can be used in routine variety discrimination. With three enzymes combined (PGI, ACP, IDH) and two buffer systems, all the varieties tested could be distinguished from one another."
Note:Pictures, b/w
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Greneche, M., J. Lallemand, and O. Michaud. 1991. Comparison of different enzyme loci as a means of distinguishing ryegrass varieties by electrophoresis. Seed Sci. Technol. 19(1):p. 147-158.
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