Full TGIF Record # 18937
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Author(s):Welty, R. E.; Milbrath, G. M.; Faulkenberry, D.; Azevedo, M. D.; Meek, L.; Hall, K.
Author Affiliation:Agricultural Research Service, US Department of Agriculture, Oregon State University, Corvallis, OR
Title:Endophyte detection in tall fescue seed by staining and ELISA
Source:Seed Science and Technology. Vol. 14, No. 1, 1986, p. 105-115.
Publishing Information:International Seed Testing Association
Keywords:TIC Keywords: Acremonium coenophialum; Festuca arundinacea; Endophytic fungi
Abstract/Contents:"Acremonium coenophialum, an endophyte of cool-season grasses, is a seedborne fungus in tall fescue(Festuca arundinacea). Cattle grazing plants infected with the endophyte suffer from a disorder known as summer syndrome. The fungus is spread by planting infected seed. Detection of the fungus by staining and microscopic examination was compared with detection by an enzyme-linked immunosorbent assay(ELISA). Both methods were satisfactory in identifying seed lots with high levels of endophyte infection(30% or more). Seed staining separated seed lots with 0% from those with 5% infected seeds. An ELISA detection threshold point (absorbance at 405 nm) could not be consistently established for seed lots with 0-10% infected seeds. ELISA gave false positive readings on several seed lots when microscopic examination revealed no infection. When procedures involving single, double, and sequential sampling of seed lots were compared, the accuracy of the three procedures was similar. Fewer seeds were needed using sequential sampling for acceptance of a seed lot containing 5% or fewer infected seeds. Microscopic examination of stained seed using sequential samples is recommended for detecting low levels(0-10%) of infection by A. coenophialum in tall fescue seed."
Note:Pictures, b/w
Summary in French and German
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Welty, R. E., G. M. Milbrath, D. Faulkenberry, M. D. Azevedo, L. Meek, and K. Hall. 1986. Endophyte detection in tall fescue seed by staining and ELISA. Seed Sci. Technol. 14(1):p. 105-115.
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