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| Web URL(s): | https://www.jstor.org/stable/23433608?seq=1
Last checked: 04/15/2016
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https://www.jstor.org/stable/pdf/23433608.pdf
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Chandra-Shekara, A.C.;
Thompson, Michael;
Schultz, Quentin;
Venkatramana, Pegadaraju |
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| Author Affiliation: | BioDiagnostics Inc., River Falls, WI |
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| Title: | Bonafide BDITM - Pure PRG; A novel alternative to ryegrass seedling root fluorescence test |
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| Source: | Seed Technology. Vol. 32, No. 2, 2010, p. 149. |
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| Publishing Information: | Lincoln, Nebraska: Association of Official Seed Analysts and Society of Commercial Seed Technologists |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Lolium multiflorum; Polymerase chain reaction; Seed industry; Seed purity; Seed testing; Fluorescence test
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| Abstract/Contents: | "Adventitious presence (unintended presence) of annual ryegrass in perennial ryegrass seed lots causes significant economic losses to grass seed industry. International Seed Testing Association (ISTA) recommends the usage of SRF (Seedling Root Fluorescence) and/or grow out test procedures to estimate the levels of annual rye presence in perennial seed lots. However, both SRF and growout test are labor intensive and time consuming. Furthermore, the SRF test produces inaccurate results and is environmentally influenced. Increasing numbers of perennial seed lots are rejected each year due to the inaccuracy of the SRF test. Thus, there is a clear need for a better testing procedure that could meet the diagnostic needs for ryegrass in an efficacious, rapid & cost effective manner. Towards this end we have developed a high throughput quantitative PCR (Q-PCR) based diagnostic tool that effectively detects the presence of annual rye grass seed contamination in a perennial rye grass lots. The DNA test is designed using an insertion/deletion (In-Del) site in a ryegrass gene involved in regulating the vernalization response of ryegrass. This new DNA test is more sensitive, accurate and cost effective in detecting annual and intermediate type contamination in perennial ryegrass with a high sensitivity of 0.04% in a sample size of 5000 seeds. Use of larger sample sizes (12.5-fold higher compared to SRF test) provided additional accuracy in detecting the level of contamination. This method has been validated on 68 perennial, 26 annual and 14 intermediate ryegrass varieties with consistent results." |
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| Language: | English |
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| References: | 0 |
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| Note: | "Abstracts from oral and poster presentations given at the 100th Association of Official Seed Analysts and the 87th Society of Commercial Seed Technologists (AOSA/SCST) Annual Meeting held in St. Louis, Missouri on June 7-10, 2010"
This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Chandra-Shekara, A.C., M. Thompson, Q. Schultz, and P. Venkatramana. 2010. Bonafide BDITM - Pure PRG; A novel alternative to ryegrass seedling root fluorescence test. Seed Technology. 32(2):p. 149. |
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| Web URL(s):
https://www.jstor.org/stable/23433608?seq=1
Last checked: 04/15/2016
Access conditions: Item is within a limited-access website
https://www.jstor.org/stable/pdf/23433608.pdf
Last checked: 04/15/2016
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b3628131 |
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