Full TGIF Record # 19208
Item 1 of 1
Publication Type:
i
Report
Author(s):Redmond, Carl T.; Potter, Daniel A.
Title:Comparative infectivity of in vitro and in vivo forumlations of Bacillus popilliae, casual agent of milky disease, for biological control of Japanese beetle grubs
Source:Kentucky Turfgrass Research. 1990, p. 31-33.
Publishing Information:Lexington, KY: University of Kentucky, College of Agriculture, Cooperative Extension Service
Series:Progress Report 328
Keywords:TIC Keywords: Milky disease; Popillia japonica; Bacillus popilliae; Grubs; Formulations; In Vitro; Spores; Infection; Biological pest control; Lolium perenne; Trifolium repens; Biological control
Abstract/Contents:Compares ability of in vivo and in vitro milky disease spore formulations to infect and reduce Japanese beetle grub populations. In 2 field studies, incidence of milky disease and grub survival was studied in large plots (4m x 4m) and hoop enclosures (0.1m2). Treatments were untreated, Fairfax in vivo poweder, Reuter in vitro powder, and Reuter in vitro granular. Plots were treated in early September 1988. Grub survival in late May and early October 1989 is reported in tables. There was no significant reduction in JB grub numbers and no increase in the incidence of MD in the grub population in either experiment. Sampling will continue in these experiments for two more years. In 2 lab studies incidence of milky disease and grub survival was studied, grubs held in individual containers exposed to dosages up to 100 times higher than field rated or in replicated groups with different dosage rates fed soil/spore mixtures for 45 days before. In both of the experiments, the traditional in vivo spore formulation had a significantly higher infectivity rate than the new in vitro spore formulation, however the maximum infectivity rate for any of the dosages was only 32 percent. Neither formulation caused significant mortality of JB grubs at any dosages during the experimental period. Additional experiments are being conducted to determine the effects of MD on feeding and activity of diseased grubs. These experiments and further results of the long-term investigations will be summarized in upcoming reports.
Language:English
References:0
Note:Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Redmond, C. T., and D. A. Potter. 1990. Comparative infectivity of in vitro and in vivo forumlations of Bacillus popilliae, casual agent of milky disease, for biological control of Japanese beetle grubs. KY. Turfgrass Res. p. 31-33.
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