Full TGIF Record # 193190
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DOI:10.1094/PDIS-01-11-0073
Web URL(s):http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-01-11-0073
    Last checked: 11/16/2011
    Requires: PDF Reader
Publication Type:
i
Refereed
Author(s):Beirn, Lisa A.; Moy, Melinda; Meyer, William A.; Clarke, Bruce B.; Crouch, Jo Anne
Author Affiliation:Beirn, Moy, Meyer, and Clarke: Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ; Crouch: Agricultural Research Service, U.S. Department of Agriculture, Systematic Mycology and Microbiology Laboratory, Beltsville, MD
Title:Molecular analysis of turfgrass rusts reveals the widespread distribution of Puccinia coronata as a pathogen of Kentucky bluegrass in the United States
Source:Plant Disease. Vol. 95, No. 12, December 2011, p. 1547-1557.
Publishing Information:St. Paul, MN: American Phytopathological Society
# of Pages:11
Related Web URL:http://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-01-11-0073
    Last checked: 11/16/2011
    Notes: Abstract only
http://digitalgcm.gcsaa.org/?id=32&pageid=109
    Last checked: 01/10/2012
    Requires: Microsoft Silverlight or Adobe Flash (user default)
    Notes: Document is located within a single large file; Golf Course Management version
Keywords:TIC Keywords: Cultivar susceptibility; Diagnostic techniques; Disease identification; Host plant resistance; Molecular biology; Pigmentation; Poa pratensis; Puccinia coronata var. coronata; Rusts; Spread of disease
Abstract/Contents:"Over the past 10 years, rust diseases have become increasingly prevalent on certain cultivars of Kentucky bluegrass. This pattern suggests that new races or new species of rust fungi may have emerged. To test this hypothesis, 66 samples of turfgrass rust fungi collected from across the United States were evaluated based on sequences of the internal transcribed spacer (ITS)-5.8S rDNA region. Phylogenetic analysis revealed three species: Puccinia coronata, P. graminis, and P. striiformis, comprising 67, 28, and 5% of the samples, respectively. P. coronata was frequently found in association with Kentucky bluegrass, a host-pathogen relationship that has not been previously reported. Comparison of molecular analyses with the use of standard field identification techniques–host association and pustule pigmentation–showed that 58% of the Kentucky bluegrass samples would have been incorrectly diagnosed using nonmolecular criteria. To avoid such misidentifications, a real-time polymerase chain reaction diagnostic protocol was developed for turfgrass-associated P. graminis, P. coronata, and P. striiformis using ITS sequences. Accurate, reproducible, species-specific identifications were made using as few as 50 to 150 urediniospores, even in mixed infections. This study represents the first DNA-based evaluation of turfgrass rust fungi and provides a quick and reliable sequence-based protocol as an alternative to less reliable field-based identification techniques."
Language:English
References:45
See Also:See also related summary "Molecular analysis of turfgrass rusts reveals the widespread distributed of Puccinia coronata as a pathogen of Kentucky bluegrass in the United States" Phytopathology, 100(65) June 2010, p. S13, R=172526 R=172526
Note:Partial reprint appears in Golf Course Management, 80(1) January 2012, p. 107-108
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Beirn, L. A., M. Moy, W. A. Meyer, B. B. Clarke, and J. A. Crouch. 2011. Molecular analysis of turfgrass rusts reveals the widespread distribution of Puccinia coronata as a pathogen of Kentucky bluegrass in the United States. Plant Disease. 95(12):p. 1547-1557.
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DOI: 10.1094/PDIS-01-11-0073
Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-01-11-0073
    Last checked: 11/16/2011
    Requires: PDF Reader
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