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DOI: | 10.1094/PDIS-01-11-0073 |
Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-01-11-0073 Last checked: 11/16/2011 Requires: PDF Reader |
Publication Type:
| Refereed |
Author(s): | Beirn, Lisa A.;
Moy, Melinda;
Meyer, William A.;
Clarke, Bruce B.;
Crouch, Jo Anne |
Author Affiliation: | Beirn, Moy, Meyer, and Clarke: Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ; Crouch: Agricultural Research Service, U.S. Department of Agriculture, Systematic Mycology and Microbiology Laboratory, Beltsville, MD |
Title: | Molecular analysis of turfgrass rusts reveals the widespread distribution of Puccinia coronata as a pathogen of Kentucky bluegrass in the United States |
Source: | Plant Disease. Vol. 95, No. 12, December 2011, p. 1547-1557. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
# of Pages: | 11 |
Related Web URL: | http://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-01-11-0073 Last checked: 11/16/2011 Notes: Abstract only http://digitalgcm.gcsaa.org/?id=32&pageid=109 Last checked: 01/10/2012 Requires: Microsoft Silverlight or Adobe Flash (user default) Notes: Document is located within a single large file; Golf Course Management version |
Keywords: | TIC Keywords: Cultivar susceptibility; Diagnostic techniques; Disease identification; Host plant resistance; Molecular biology; Pigmentation; Poa pratensis; Puccinia coronata var. coronata; Rusts; Spread of disease
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Abstract/Contents: | "Over the past 10 years, rust diseases have become increasingly prevalent on certain cultivars of Kentucky bluegrass. This pattern suggests that new races or new species of rust fungi may have emerged. To test this hypothesis, 66 samples of turfgrass rust fungi collected from across the United States were evaluated based on sequences of the internal transcribed spacer (ITS)-5.8S rDNA region. Phylogenetic analysis revealed three species: Puccinia coronata, P. graminis, and P. striiformis, comprising 67, 28, and 5% of the samples, respectively. P. coronata was frequently found in association with Kentucky bluegrass, a host-pathogen relationship that has not been previously reported. Comparison of molecular analyses with the use of standard field identification techniques–host association and pustule pigmentation–showed that 58% of the Kentucky bluegrass samples would have been incorrectly diagnosed using nonmolecular criteria. To avoid such misidentifications, a real-time polymerase chain reaction diagnostic protocol was developed for turfgrass-associated P. graminis, P. coronata, and P. striiformis using ITS sequences. Accurate, reproducible, species-specific identifications were made using as few as 50 to 150 urediniospores, even in mixed infections. This study represents the first DNA-based evaluation of turfgrass rust fungi and provides a quick and reliable sequence-based protocol as an alternative to less reliable field-based identification techniques." |
Language: | English |
References: | 45 |
See Also: | See also related summary "Molecular analysis of turfgrass rusts reveals the widespread distributed of Puccinia coronata as a pathogen of Kentucky bluegrass in the United States" Phytopathology, 100(65) June 2010, p. S13, R=172526 R=172526 |
Note: | Partial reprint appears in Golf Course Management, 80(1) January 2012, p. 107-108 Figures Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Beirn, L. A., M. Moy, W. A. Meyer, B. B. Clarke, and J. A. Crouch. 2011. Molecular analysis of turfgrass rusts reveals the widespread distribution of Puccinia coronata as a pathogen of Kentucky bluegrass in the United States. Plant Disease. 95(12):p. 1547-1557. |
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| DOI: 10.1094/PDIS-01-11-0073 |
| Web URL(s): http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-01-11-0073 Last checked: 11/16/2011 Requires: PDF Reader |
| MSU catalog number: b2194824a |
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