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Publication Type:
| Refereed |
Author(s): | Van Dreven, F.;
Esselink, G.;
Houwing, A. |
Author Affiliation: | Centre of Variety Research and Seed Technology (CRZ), Wageningen, The Netherlands |
Title: | Esterase isoenzyme differences between cultivars of Kentucky bluegrass (Poa pratensis L.) from seed extracts using isoelectric focusing |
Source: | Plant Varieties and Seeds. Vol. 3, No. 2, July 1990, p. 89-97. |
Publishing Information: | Oxford: Blackwell Scientific Publications |
Keywords: | TIC Keywords: Isoenzymes; Electophoresis; Poa pratensis
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Abstract/Contents: | "Seed proteins from 122 registered cultivars of Kentucky bluegrass (Poa pratensis L.) were analysed for variation of the isoenzyme esterase using isoelectric focusing (IEF) of bulk seed samples. After electrophoresis in IEF gels of pH 3-10, cultivars could be classified into six categories, using only the first band near the cathode for discrimination. This classification was only slightly related to morphological characteristics. Using the whole banding pattern for comparison, 102 cultivars could be discriminated in total. Samples of a cultivar from different harvest years showed the same banding pattern. By using bulk seed samples, a mixture of cultivars could wrongly be interpreted as a new cultivar, since homogeneity of a cultivar could not be observed. For examining distinctness, uniformity and stability (DUS) of a cultivar, it could be useful to combine electrophoresis results with morphological characteristics." |
Language: | English |
References: | 22 |
Note: | Figures Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Van Dreven, F., G. Esselink, and A. Houwing. 1990. Esterase isoenzyme differences between cultivars of Kentucky bluegrass (Poa pratensis L.) from seed extracts using isoelectric focusing. Plant Var. Seeds. 3(2):p. 89-97. |
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