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| Web URL(s): | http://usgatero.msu.edu/v10/n22.pdf
Last checked: 12/1/2011
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Author(s): | Richmond, Douglas S.;
Schemerhorn, Brandi J.;
Abdelwahab, Mohamed G. |
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| Author Affiliation: | Richmond: Associate Professor; Schemerhorn: Adjunct Assistant Professor; Abdelwahab: Post-Doctoral Scientist, Department of Entomology, Purdue University, West Lafayette, IN |
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| Title: | Developing a DNA-based larval identification tool for billbugs |
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| Source: | USGA Turfgrass and Environmental Research Online. Vol. 10, No. 22, November 15 2011, p. [1-7]. |
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| Publishing Information: | Far Hills, NJ: United States Golf Association, Green Section |
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| # of Pages: | 9 |
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| Keywords: | TIC Keywords: Evaluative methods; Identification keys; Insect control; Insect identification; Insect profile; Larva; Molecular markers; Sphenophorus
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| Abstract/Contents: | "Billbugs pose a serious threat to managed turfgrass across North America. With the continuously expanding ranges of several species resulting in novel species interactions and a nation-wide collage of billbug species assemblages, satisfactory management has been difficult to achieve in many regions. Because the damaging larval stages of these insects cannot be identified to species, the seasonal population dynamics of many of the most common billbug pests remains largely unresolved. This project aimed to explore the potential for exploiting the unique properties of the nuclear ribosomal DNA multigene family (nrDNA) to identify species-specific molecular markers that can be used in diagnostic assays for several of the most common billbug species associated with turfgrass environments. Results to date include: Two nrDNA internal transcribed spacer regions (ITS1 and ITS2) were selected as the focus of efforts to differentiate 6 common billbug species (Sphenophorus venatus, S. parvulus, S. minimus, S. inaequalis, S .cicatristriatus, and S. phoenesiensis) associated with managed turfgrass. ITS1 and ITS2 each provided adequate variation in size and nucleotide sequence to allow for complete differentiation of the species examined based on either nrDNA region. Using these sequences, it should be possible to design specific primers that will facilitate identification of the otherwise indistinguishable larval stage of these insects based on only a single nrDNA region (ITS1 or ITS2). These findings could greatly facilitate the collection of baseline information on billbug seasonal ecology that is needed in order to formulate and improve management strategies and clarify new management opportunities." |
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| Language: | English |
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| References: | 12 |
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| Note: | Summary as abstract
Pictures, color
Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Richmond, D. S., B. J. Schemerhorn, and M. G. Abdelwahab. 2011. Developing a DNA-based larval identification tool for billbugs. USGA Turfgrass Environ. Res. Online. 10(22):p. [1-7]. |
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| Web URL(s):
http://usgatero.msu.edu/v10/n22.pdf
Last checked: 12/1/2011
Requires: PDF Reader |
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MSU catalog number: b3952822a |
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