| |
| DOI: | 10.21273/JASHS.136.6.399 |
|---|
| Web URL(s): | https://journals.ashs.org/jashs/view/journals/jashs/136/6/article-p399.xml
Last checked: 04/30/2020
https://journals.ashs.org/jashs/downloadpdf/journals/jashs/136/6/article-p399.xml
Last checked: 04/30/2020
Requires: PDF Reader |
|---|
| Publication Type:
| Refereed |
|---|
| Author(s): | Xu, Chenping;
Jiang, Zhongchun;
Huang, Bingru |
|---|
| Author Affiliation: | Xu and Huang: Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ; Jiang: Department of Plant Science, State University of New York, Cobleskill, NY |
|---|
| Title: | Nitrogen deficiency-induced protein changes in immature and mature leaves of creeping bentgrass |
|---|
| Section: | Environmental stress physiology
Other records with the "Environmental stress physiology" Section
|
|---|
| Source: | Journal of The American Society for Horticultural Science. Vol. 136, No. 6, November 2011, p. 399-407. |
|---|
| Publishing Information: | Alexandria, Va.: American Society for Horticultural Science |
|---|
| # of Pages: | 9 |
|---|
| Keywords: | TIC Keywords: Agrostis stolonifera; Antioxidants; Comparisons; Fertilization rates; Gel electrophoresis; Heat shock proteins; Nitrogen deficiency; Photorespiration; Plant growth regulators; Plant metabolism; Plant proteins; Senescence
|
|---|
| Cultivar Names: | Penncross |
|---|
| Abstract/Contents: | "Nitrogen (N) deficiency inhibits plant growth and induces leaf senescence through regulating various metabolic processes. The objectives of this study were to examine protein changes in response to N deficiency in immature and mature leaves of a perennial grass species and determine major metabolic processes affected by N deficiency through proteomic profiling. Creeping bentgrass (Agrostis stolonifera cv. Penncross) plants were originally fertilized with a diluted 36N-2.6P-5K fertilizer. After 14 days acclimation in a growth chamber, plants were grown in a nutrient solution containing 6 mm nitrate (control) or without N (N deficiency). Immature leaves (upper first and second not yet fully expanded leaves) and mature leaves Antioxidants(lower fully expanded leaves) were separated at 28 days of treatment for protein analysis. Two-dimensional electrophoresis and mass spectrometry analysis were used to identify protein changes in immature and mature leaves in response to N deficiency. The abundance of many proteins in both immature and mature leaves decreased with N deficiency, including those involved in photosynthesis, photorespiration, and amino acid metabolism (hydroxypyruvate reductase, serine hydroxymethyltransferase, alanine aminotransferase, glycine decarboxylase complex, glycolate oxidase), protein protection [heat shock protein (HSP)/HSP 70, chaperonin 60 and FtsH-like protein], and RNA stability (RNA binding protein). The reduction in protein abundance under N deficiency was greater in mature leaves than in immature leaves. The abundance of small HSP and metalloendopeptidase increased under N deficiency only in immature leaves. These results suggest that N deficiency accelerated protein degradation in immature and mature leaves of creeping bentgrass, particularly those proteins associated with energy and metabolism, but to a lesser extent in immature leaves. Immature leaves were also able to accumulate proteins with chaperone functions and for N reutilization, which could protect leaves from senescence under N deficiency." |
|---|
| Language: | English |
|---|
| References: | 46 |
|---|
| Note: | Figures
Tables |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Xu, C., Z. Jiang, and B. Huang. 2011. Nitrogen deficiency-induced protein changes in immature and mature leaves of creeping bentgrass. J. Am. Soc. Hortic. Sci. 136(6):p. 399-407. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=194975 |
| If there are problems with this record, send us feedback about record 194975. |
| Choices for finding the above item: |
|---|
| DOI: 10.21273/JASHS.136.6.399 |
| Web URL(s):
https://journals.ashs.org/jashs/view/journals/jashs/136/6/article-p399.xml
Last checked: 04/30/2020
https://journals.ashs.org/jashs/downloadpdf/journals/jashs/136/6/article-p399.xml
Last checked: 04/30/2020
Requires: PDF Reader |
| 
MSU catalog number: b2221587a |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
