Full TGIF Record # 197200
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Web URL(s):http://turf.rutgers.edu/research/abstracts/symposium2012.pdf#page=33
    Last checked: 02/07/2012
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Publication Type:
i
Report
Content Type:Abstract or Summary only
Author(s):Ambrose, Karen; Belanger, Faith
Author Affiliation:Department of Plant Biology and Pathology, Rutgers University
Title:The application of next-generation sequencing technology is helping us elucidate the genetics of turfgrass-endophyte symbiotic interaction
Section:Poster presentations
Other records with the "Poster presentations" Section
Meeting Info.:New Brunswick, New Jersey: January 6, 2012
Source:Proceedings of the Twenty-First Annual Rutgers Turfgrass Symposium. 2012, p. 32.
Publishing Information:New Brunswick, NJ: Center for Turfgrass Science, Rutgers, The State University of New Jersey
# of Pages:1
Keywords:TIC Keywords: Endophytes; Epichloe festucae; Festuca rubra; Genetic analysis; Genetic structure; Symbiosis
Abstract/Contents:"Our research aims to gain insight into the biological process of plant-microbe symbiotic interactions using high-throughput DNA sequencing. Primarily, we are interested in how the plant's gene expression profile is impacted by the presence of a naturally occuring fungal endophyte. Symbiosis between fungi and plants in nature is well documented. Yet, much research is still needed to fully comprehend the workings of this important interaction in regards to plant evolution, breeeding and physiological performance. One such symbiotic association of interest takes place between Festuca rubra, a commercially important grass species known as fine fescue, and Epichloë festucae, a fungal endophyte that lives within its grass host. Studies have revealed that Epichloë endophytes live entirely within the plant, and provide a wide range of benefits to their grass hosts, principally resitsnce to insect and mammalian herbivores. E. festucae infection of F. rubra also confers fungal disease resistance to the host. These observations are important because resistance to diseases has not been documented in other cool-season grass-endophyte interactions. We have utilized transcriptome analysis to address fundamental aspects of plantendophyte symbiosis in order to facilitate large-scale comparative analysis. Based on our hypothesis that differential gene expression is imperative for the maintenance of symbiotic interaction, we have used SAGE (Serial Analysis of Gene Expression) to obtain a global quantitative comparison of the transcriptome of the endophyte-free (E-) and endophyte-infected (E+) plants. The SAGE libraries were prepared in triplicates, and sequenced using ABI's SOLiD platform. The >54 million SAGE tags we obtained were supplemented with >200,000,454 transcriptome sequences of the E. festucae endophyte strain isolated froom its grass host plus the plant infected with the strain. The SAGE tags were mapped to a reference database consisting of our 454 transcriptome, and Festuca and Epichloë sequences downloaded from NCBI. On going analysis of the data has revealed plant transcriptional changes in response to fungal endophyte infection. Ultimately, the findings will enrich our overall understanding of how symbiotic relationships in nature, a complex interplay between host plants and thier microbe symbionts, are maintained. Specifically, the research will contribute to the knowledge of the symbiosis between a grass host plant and its fungal endophyte by identifying the important genes responsible for maintaining the equilibrium, where both plant and endophyte thrive."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Ambrose, K., and F. Belanger. 2012. The application of next-generation sequencing technology is helping us elucidate the genetics of turfgrass-endophyte symbiotic interaction. Proc. Rutgers Turfgrass Symp. p. 32.
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http://turf.rutgers.edu/research/abstracts/symposium2012.pdf#page=33
    Last checked: 02/07/2012
    Requires: PDF Reader
    Notes: Item is within a single large file
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