Full TGIF Record # 198797
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Publication Type:
i
Report
Content Type:Abstract or Summary only
Author(s):Barker, Reed E.; Barker, Judy
Author Affiliation:Grass Genomic Testing, Inc., Salem, OR
Title:Genetic testing in ryegrass: Beyond visual inspection
Section:Abstracts
Other records with the "Abstracts" Section
Source:Seed Technology. Vol. 33, No. 2, 2011, p. 201.
Publishing Information:Lincoln, Nebraska: Association of Official Seed Analysts and Society of Commercial Seed Technologists
# of Pages:1
Keywords:TIC Keywords: Evaluations; Fluorescence test; Genetic analysis; Lolium; Seed contamination
Abstract/Contents:"Seedling root fluorescence (SRF) of ryegrass has been used in seed testing almost since it was discovered in 1929. The SRF test is used to predict contamination of annual ryegrass in perennial ryegrass seed lots. The test is easy to conduct, but quite often provides wrong results because false positives are observed. While warnings that SRF should only be used as general indicator of ryegrass crop quality have been suggested in Oregon since 1938, the test has become entrenched in seed testing as an absolute estimator of contamination. Seed growers' payments have been docked and end use customers provided an inadequately tested product as a result of this poorly applied test. The maturity grow-out test (GOT) was developed to provide a better estimate of plant type and reduce the dockage problem for growers. To promote uniformity, testing conditions for the GOT must be the same in each laboratory that conducts the test and these conditions are hard to standardize. The GOT takes at least 42 days to complete, but the subjective end point determination makes it difficult to identify seedlings that actually have an annual or perennial growth type. The GOT is a measure of vernalization (flowering response after cold treatment) requirement and the actual test length for this trait, as in barley and wheat, should be 100 days. Nucleic acid tests (PCR) on genomic DNA have been developed that determine the presence or absence of the actual alleles (alternate forms of genes) involved in flowering control of grasses. Conducting these chemicals tests is beyond visual inspection, but prediction of the potential growth type can be done more quickly, more accurately, and provide more uniform results on a per plant basis than is possible with GOT."
Language:English
References:0
Note:This item is an abstract only!
"Abstracts from oral and poster presentations given at the 101st Association of Official Seed Analysts and the 88th Society of Commercial Seed Technologists (AOSA/SCST)"
"meeting: Williamsburg, Virginia: June 7-10, 2011"
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Barker, R. E., and J. Barker. 2011. Genetic testing in ryegrass: Beyond visual inspection. Seed Technology. 33(2):p. 201.
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Web URL(s):
https://www.jstor.org/stable/23433431?seq=1
    Last checked: 04/15/2016
    Access conditions: Item is within a limited-access website
https://www.jstor.org/stable/pdf/23433431.pdf
    Last checked: 04/15/2016
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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