Full TGIF Record # 198879
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DOI:10.1111/j.1469-8137.2005.01345.x
Web URL(s):http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2005.01345.x/full
    Last checked: 03/02/2012
http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2005.01345.x/pdf
    Last checked: 03/02/2012
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Publication Type:
i
Refereed
Author(s):Guo, Yangdong; Mizukami, Yuko; Yamada, Toshihiko
Author Affiliation:Guo and Yamada: National Agricultural Research Center for Hokkaido Region, Sapporo, Japan; Guo: China Agricultural University, Beijing, China; Mizukami: Aichi Agricultural Research Center, Yazako, Nagakute, Aichi, Japan
Title:Genetic characterization of androgenic progeny derived from Lolium perenne X Festuca pratensis cultivars
Source:New Phytologist. Vol. 166, No. 2, May 2005, p. 455-464.
Publishing Information:Oxford, England: Blackwell Publishing, for the New Phytologist Trust
# of Pages:10
Related Web URL:http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2005.01345.x/abstract
    Last checked: 03/12/2012
    Notes: Abstract only
Keywords:TIC Keywords: Anthers; Chromosome pairing; Cold resistance; Cultivar improvement; Fertility; Festuca arundinacea; Festuca pratensis; Festulolium; Genetic characterization; Genetic variability; Fluorescence in situ hybridization; Lolium multiflorum; Lolium perenne; Pollen
Abstract/Contents:"Androgenesis and genetic characterization of androgenic progeny from Lolium perenne X Festuca pratensis cultivars (2n = 4x = 28) were investigated in order to develop a novel grass that combines favourable attributes of parent plants. A successful androgenesis was obtained using PG-96 medium. The green plant regeneration was 46%, 35% and 17% for Bx350, Bx351 and Prior, respectively, and over 800 green plants have been obtained. Androgenic progeny showed a large variation in freezing tolerance, 7% of 292 progeny exceeding that of freezing hardy F. pratensis despite containing chromosomes of L. perenne. More than 60% of 175 flowering progeny produced dehiscent anthers with pollens. Androgenic plants contained 14 or 28 chromosomes. There were 188 (56%), 204 (77%) and 114 (81%) dihaploids from Bx350, Bx351 and Prior, respectively. The nuclear DNA content varied significantly even between plants with the same chromosome number. High levels of chromosome pairing and recombination were observed by genomic in situ hybridization analysis because of close homology between two genomes. The results indicated that androgenic progeny of Festulolium showed high level of genetic variation, and provide potential for accelerating selection efficiency."
Language:English
References:36
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Guo, Y., Y. Mizukami, and T. Yamada. 2005. Genetic characterization of androgenic progeny derived from Lolium perenne X Festuca pratensis cultivars. New Phytol. 166(2):p. 455-464.
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DOI: 10.1111/j.1469-8137.2005.01345.x
Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2005.01345.x/full
    Last checked: 03/02/2012
http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2005.01345.x/pdf
    Last checked: 03/02/2012
    Requires: PDF Reader
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