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| DOI: | 10.1007/s00122-004-1870-y |
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| Web URL(s): | https://link.springer.com/article/10.1007%2Fs00122-004-1870-y
Last checked: 10/05/2017 |
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| Publication Type:
| Refereed |
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| Author(s): | Donnison, Iain S.;
O'Sullivan, Donal M.;
Thomas, Ann;
Canter, Peter;
Moore, Beverley;
Armstead, Ian;
Thomas, Howard;
Edwards, Keith J.;
King, Ian P. |
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| Author Affiliation: | Donnison, A. Thomas, Canter, Moore, Armstead, H. Thomas and King: Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, UK; O'Sullivan and Edwards: IACR-LARS, Long Ashton, Bristol, UK |
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| Title: | Construction of a Festuca pratensis BAC library for map-based cloning in Festulolium substitution lines |
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| Source: | TAG: Theoretical and Applied Genetics. Vol. 110, No. 5, March 2005, p. 846-851. |
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| Publishing Information: | Berlin: Springer |
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| # of Pages: | 6 |
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| Related Web URL: | http://www.springerlink.com/content/?k=pub%3a%28Theoretical+and+applied+and+genetics%29+vol%3a%28110%29+iss%3a%285%29+p%3a%28846%29
Last checked: 03/16/2012
Notes: Abstract only |
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| Keywords: | TIC Keywords: Amplification fragment length polymorphisms; Festuca pratensis; Festulolium; Lolium perenne
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| Abstract/Contents: | "Introgression in Festulolium is a potentially powerful tool to isolate genes for a large number of traits which differ between Festuca pratensis Huds. and Lolium perenne L. Not only are hybrids between the two species fertile, but the two genomes can be distinguished by genomic in situ hybridisation and a high frequency of recombination occurs between homoeologous chromosomes and chromosome segments. By a programme of introgression and a series of backcrosses, L. perenne lines have been produced which contain small F. pratensis substitutions. This material is a rich source of polymorphic markers targeted towards any trait carried on the F. pratensis substitution not observed in the L. perenne background. We describe here the construction of an F. pratensis BAC library, which establishes the basis of a map-based cloning strategy in L. perenne. The library contains 49,152 clones, with an average insert size of 112 kbp, providing coverage of 2.5 haploid genome equivalents. We have screened the library for eight amplified fragment length polymorphism (AFLP) derived markers known to be linked to an F. pratensis gene introgressed into L. perenne and conferring a staygreen phenotype as a consequence of a mutation in primary chlorophyll catabolism. While for four of the markers it was possible to identify bacterial artificial chromosome (BAC) clones, the other four AFLPs were too repetitive to enable reliable identification of locus-specific BACs. Moreover, when the four BACs were partially sequenced, no obvious coding regions could be identified. This contrasted to BACs identified using cDNA sequences, when multiple genes were identified on the same BAC." |
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| Language: | English |
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| References: | 23 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Donnison, I. S., D. M. O'Sullivan, A. Thomas, P. Canter, B. Moore, I. Armstead, et al. 2005. Construction of a Festuca pratensis BAC library for map-based cloning in Festulolium substitution lines. Theor. Appl. Genet. 110(5):p. 846-851. |
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| DOI: 10.1007/s00122-004-1870-y |
| Web URL(s):
https://link.springer.com/article/10.1007%2Fs00122-004-1870-y
Last checked: 10/05/2017 |
| 
MSU catalog number: b2203373 |
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