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| DOI: | 10.1007/s00122-004-1785-7 |
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| Web URL(s): | https://link.springer.com/content/pdf/10.1007%2Fs00122-004-1785-7.pdf
Last checked: 10/05/2017
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https://link.springer.com/article/10.1007/s00122-004-1785-7/fulltext.html
Last checked: 10/05/2017
Access conditions: Item is within a limited access website |
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| Publication Type:
| Refereed |
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| Author(s): | Faville, M. J.;
Vecchies, A. C.;
Schreiber, M.;
Drayton, M. C.;
Hughes, L. J.;
Jones, E. S.;
Guthridge, K. M.;
Smith, K. F.;
Sawbridge, T.;
Spangenberg, G. C.;
Bryan, G. T.;
Forster, J. W. |
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| Author Affiliation: | Faville and Bryan: Grasslands Research Centre, AgResearch Ltd., Palmerston North, New Zealand; Vecchies, Drayton, Hughes, Jones, Guthridge, Sawbridge, Spangenberg and Forster: Primary Industries Research Victoria, Plant Biotechnology Centre, La Trobe University, Bundoora, VIC, Australia and Molecular Plant Breeding Cooperative Research Centre, Australia; Schreiber: AgResearch Limited, School of Biological Science, University of Auckland, Auckland, New Zealand; Smith: Molecular Plant Breeding Cooperative Research Centre, Australia and Primary Industries Research Victoria, Hamilton Centre, Hamilton, VIC, Australia |
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| Title: | Functionally associated molecular genetic marker map construction in perennial ryegrass (Lolium perenne L.) |
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| Source: | TAG: Theoretical and Applied Genetics. Vol. 110, No. 1, December 2004, p. 12-32. |
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| Publishing Information: | Berlin: Springer |
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| # of Pages: | 21 |
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| Related Web URL: | http://link.springer.com/article/10.1007%2Fs00122-004-1785-7
Last checked: 04/29/2013
Notes: Abstract only |
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| Keywords: | TIC Keywords: Expressed sequence tag; Gene mapping; Genetic linkage; Genetic markers; Lolium perenne; Simple sequence repeats
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| Cultivar Names: | Aurora |
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| Abstract/Contents: | "A molecular marker-based map of perennial ryegrass (Lolium perenne L.) has been constructed through the use of polymorphisms associated with expressed sequence tags (ESTs). A pair-cross between genotypes from a North African ecotype and the cultivar Aurora was used to generate a two-way pseudo-testcross population. A selection of 157 cDNAs assigned to eight different functional categories associated with agronomically important biological processes was used to detect polymorphic EST-RRFLP loci in the F1(NA6 AU6) population. A comprehensive set of ESTbullSSR markers was developed from the analysis of 14,767 unigenes, with 310 primer pairs showing efficient amplification and detecting 113 polymorphic loci. Two parental genetic maps were produced: the NA6 genetic map contains 88 EST-RFLP and 71 EST-RSSR loci with a total map length of 963 cM, while the AU6 genetic map contains 67 EST-RFLP and 58 EST-SSR loci with a total map length of 757 cM. Bridging loci permitted the alignment of homologous chromosomes between the parental maps, and a sub-set of genomic DNA-derived SSRs was used to relate linkage groups to the perennial ryegrass reference map. Regions of segregation distortion were identified, in some instances in common with other perennial ryegrass maps. The EST-derived marker-based map provides the basis for in silico comparative genetic mapping, as well as the evaluation of co-location between QTLs and functionally associated genetic loci." |
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| Language: | English |
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| References: | 78 |
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| Note: | Correction appears in Theoretical and Applied Genetics, 110(5) March 2005, p. 992: "In Table 6, the sequences of individual forward and reverse amplification primers for selected EST-SSR loci were merged into one line in the final column."
Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Faville, M. J., A. C. Vecchies, M. Schreiber, M. C. Drayton, L. J. Hughes, E. S. Jones, et al. 2004. Functionally associated molecular genetic marker map construction in perennial ryegrass (Lolium perenne L.). Theor. Appl. Genet. 110(1):p. 12-32. |
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| DOI: 10.1007/s00122-004-1785-7 |
| Web URL(s):
https://link.springer.com/content/pdf/10.1007%2Fs00122-004-1785-7.pdf
Last checked: 10/05/2017
Requires: PDF Reader
Access conditions: Item is within limited-acess website
https://link.springer.com/article/10.1007/s00122-004-1785-7/fulltext.html
Last checked: 10/05/2017
Access conditions: Item is within a limited access website |
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MSU catalog number: b2203373 |
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