Full TGIF Record # 199649
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Web URL(s):http://www.academicjournals.org/article/article1380105119_Lee et al.pdf
    Last checked: 01/17/2014
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Publication Type:
i
Report
Author(s):Lee, Ki-Won; Choi, Gi Jun; Kim, Ki-Yong; Ji, Hee Chung; Park, Hyung Soo; Yoon, Sei Hyung; Lee, Sang-Hoon
Author Affiliation:Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, Korea
Title:High frequency plant regeneration from mature seed-derived callus of Italian ryegrass (Lolium multiflorum) cultivars
Section:Plant and agricultural technology
Other records with the "Plant and agricultural technology" Section
Source:African Journal of Biotechnology. Vol. 8, No. 24, December 15 2009, p. 6828-6833.
# of Pages:6
Publishing Information:Nairobi, Kenya: Kenya Academic Journals
Related Web URL:http://www.academicjournals.org/journal/AJB/article-abstract/BFF541211230
    Last checked: 01/17/2014
Keywords:TIC Keywords: 2,4-D; Additive genes; Calli; Cold resistance; Copper sulfate; Cultivar improvement; Embryogenesis; Growth analysis; Lolium multiflorum; Molecular genetics; Proline; Regeneration; Seeding rate; Silver nitrate
Cultivar Names:Hwasan
Abstract/Contents:"In the present study, we have developed a high-frequency plant regeneration system for Italian ryegrass via callus culture using mature seeds as explants. Optimal embryogenic callus induction was found to occur in MS medium containing 5 mg l-1 2,4-D, 0.5 mg l-1 BA, 500 mg l-1 L-proline, 1 g l-1 casein hydrolysate, 30 g 1-1 sucrose, 7 mg l-1 AgNO3, 2 mg l-1 CuSO4 and solidified with 3 g l-1 Gelrite. The highest regeneration rate was obtained in MS medium containing 1 mg l-1 2,4-D, 5 mg l-1 BA, 500 mg l-1 L-proline, 1 g l-1 casein hydrolysate, 1 mg l-1 thiamine-HCl, 30 g l-1 sucrose, 7 mg l-1 AgNO3, 2 mg l-1 CuSO4 and solidified with 3 g l-1 Gelrite. By using the most effective treatment determined for each parameter, the highest rates of embryogenic callus formation (48.9%) and regeneration (47.6%) were obtained with the Hwasan 101 cultivar. The overall plant regeneration rates of the examined cultivars ranged from 7.5 to 23.2%. Thus, optimization of regeneration frequency using mature seeds as explant material may offer a simple and efficient protocol for Italian ryegrass that may improve molecular breeding of this species."
Language:English
References:25
Note:Pictures, color
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lee, K.-W., G. J. Choi, K.-Y. Kim, H. C. Ji, H. S. Park, S. H. Yoon, et al. 2009. High frequency plant regeneration from mature seed-derived callus of Italian ryegrass (Lolium multiflorum) cultivars. Afr. J. Biotechnol.. 8(24):p. 6828-6833.
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Web URL(s):
http://www.academicjournals.org/article/article1380105119_Lee et al.pdf
    Last checked: 01/17/2014
    Requires: PDF Reader
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