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| Web URL(s): | http://www.academicjournals.org/article/article1380105119_Lee et al.pdf
Last checked: 01/17/2014
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Author(s): | Lee, Ki-Won;
Choi, Gi Jun;
Kim, Ki-Yong;
Ji, Hee Chung;
Park, Hyung Soo;
Yoon, Sei Hyung;
Lee, Sang-Hoon |
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| Author Affiliation: | Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, Korea |
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| Title: | High frequency plant regeneration from mature seed-derived callus of Italian ryegrass (Lolium multiflorum) cultivars |
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| Section: | Plant and agricultural technology
Other records with the "Plant and agricultural technology" Section
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| Source: | African Journal of Biotechnology. Vol. 8, No. 24, December 15 2009, p. 6828-6833. |
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| Publishing Information: | Nairobi, Kenya: Kenya Academic Journals |
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| # of Pages: | 6 |
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| Related Web URL: | http://www.academicjournals.org/journal/AJB/article-abstract/BFF541211230
Last checked: 01/17/2014 |
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| Keywords: | TIC Keywords: 2,4-D; Additive genes; Calli; Cold resistance; Copper sulfate; Cultivar improvement; Embryogenesis; Growth analysis; Lolium multiflorum; Molecular genetics; Proline; Regeneration; Seeding rate; Silver nitrate
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| Cultivar Names: | Hwasan |
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| Abstract/Contents: | "In the present study, we have developed a high-frequency plant regeneration system for Italian ryegrass via callus culture using mature seeds as explants. Optimal embryogenic callus induction was found to occur in MS medium containing 5 mg l-1 2,4-D, 0.5 mg l-1 BA, 500 mg l-1 L-proline, 1 g l-1 casein hydrolysate, 30 g 1-1 sucrose, 7 mg l-1 AgNO3, 2 mg l-1 CuSO4 and solidified with 3 g l-1 Gelrite. The highest regeneration rate was obtained in MS medium containing 1 mg l-1 2,4-D, 5 mg l-1 BA, 500 mg l-1 L-proline, 1 g l-1 casein hydrolysate, 1 mg l-1 thiamine-HCl, 30 g l-1 sucrose, 7 mg l-1 AgNO3, 2 mg l-1 CuSO4 and solidified with 3 g l-1 Gelrite. By using the most effective treatment determined for each parameter, the highest rates of embryogenic callus formation (48.9%) and regeneration (47.6%) were obtained with the Hwasan 101 cultivar. The overall plant regeneration rates of the examined cultivars ranged from 7.5 to 23.2%. Thus, optimization of regeneration frequency using mature seeds as explant material may offer a simple and efficient protocol for Italian ryegrass that may improve molecular breeding of this species." |
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| Language: | English |
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| References: | 25 |
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| Note: | Pictures, color
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lee, K.-W., G. J. Choi, K.-Y. Kim, H. C. Ji, H. S. Park, S. H. Yoon, et al. 2009. High frequency plant regeneration from mature seed-derived callus of Italian ryegrass (Lolium multiflorum) cultivars. Afr. J. Biotechnol.. 8(24):p. 6828-6833. |
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| Web URL(s):
http://www.academicjournals.org/article/article1380105119_Lee et al.pdf
Last checked: 01/17/2014
Requires: PDF Reader |
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MSU catalog number: b4897861 |
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