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| Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1990Articles/phyto80n10_957.pdf#page=23
Last checked: 10/15/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Tisserat, N.;
Hulbert, S.;
Nus, A. |
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| Author Affiliation: | Dep. of Plant Pathology, Kansas State University, Manhattan |
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| Title: | Identification of Leptosphaeria korrae with cloned DNA probes |
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| Source: | Phytopathology. Vol. 80, No. 10, October 1990, p. 979. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| Keywords: | TIC Keywords: Patch diseases; Disease identification; DNA
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| Abstract/Contents: | "Differentiation of Leptosphaeria korrae from other ectotrophic fungi associated with patch-type diseases of turfgrasses can be difficult because of similarities in colony morphology and the inability to induce ascocarp formation in some isolates. A more reliable means of identification was developed using two different cloned DNA fragments from L. korrae. The probes, 0.8 and 1.2 Kb in size, were specific to L. korrae, and did not hybridize to genomic DNA of other patch-disease causing fungi, including L. narmari, Gaeumannomyces incrustans, G. graminis vars. graminis, avenae, and tritici, Magnaporthe poae, Ophiosphaerella herpotricha, and Rhizoctonia solani. The DNA probes did not cross-hybridize, but hybridization patterns from EcoRI digests of genomic L. korrae DNA implied that they belonged to the same repetitive element family. No polymorphisms were detected between L. korrae isolates with either probe." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Tisserat, N., S. Hulbert, and A. Nus. 1990. Identification of Leptosphaeria korrae with cloned DNA probes. Phytopathology. 80(10):p. 979. |
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| Web URL(s):
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1990Articles/phyto80n10_957.pdf#page=23
Last checked: 10/15/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 599 .P48 |
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