Full TGIF Record # 19995
Item 1 of 1
Web URL(s):http://www.apsnet.org/publications/phytopathology/backissues/Documents/1990Articles/phyto80n10_957.pdf#page=64
    Last checked: 10/15/2015
    Requires: PDF Reader
    Notes: Item is within a single large file
Publication Type:
i
Report
Content Type:Abstract or Summary only
Author(s):Shelby, R. A.; Kelley, V. C.; Rottinghaus, G. E.
Author Affiliation:Depts. of Plant Pathology and Botany and Microbiology, Auburn University; College of Veterninary Medicine, U. of Missouri, Columbia
Title:Detection of ergovaline in fescue seed by ELISA using a monoclonal antibody
Source:Phytopathology. Vol. 80, No. 10, October 1990, p. 1020-1021.
Publishing Information:St. Paul, MN: American Phytopathological Society
Keywords:TIC Keywords: Ergovaline; Acremonium coenophialum; Endophytic fungi
Abstract/Contents:"Ergovaline (EV) is one of the ergot-peptide alkaloids of fescue infected with the endophytic fungus, Acremonium coenophialum, associated with toxicisis in grazing livestock. A monoclonal antibody to EV was used in a competitive indirect (CI) ELISA of 41 fescue seed lots. Percent fungal infection determined by microscopy varied from 0% to 99%. Accuracy of the CI ELISA in determining EV content was evaluated by HPLC. EV levels determined by both methods were positively correlated with percent infection and ranged from 0-10 micrograms/gram. CI ELISA agreed in all cases with HPLC in terms of the presence or absence of EV, indicating no false negatives or positives, but tended to underestimate EV by about one half in most samples. The CI ELISA provides a rapid screening method for presence and relative concentration of EV, to be used in conjunction with HPLC when more accurate quantitative data are needed."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Shelby, R. A., V. C. Kelley, and G. E. Rottinghaus. 1990. Detection of ergovaline in fescue seed by ELISA using a monoclonal antibody. Phytopathology. 80(10):p. 1020-1021.
Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=19995
If there are problems with this record, send us feedback about record 19995.
Choices for finding the above item:
Web URL(s):
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1990Articles/phyto80n10_957.pdf#page=64
    Last checked: 10/15/2015
    Requires: PDF Reader
    Notes: Item is within a single large file
Find Item @ MSU
MSU catalog number: SB 599 .P48
Find from within TIC:
   Digitally in TIC by record number.
Request through your local library's inter-library loan service (bring or send a copy of this TGIF record)