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| DOI: | 10.1007/s00122-006-0292-4 |
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| Web URL(s): | https://link.springer.com/article/10.1007/s00122-006-0292-4/fulltext.html
Last checked: 10/05/2017
Access conditions: Item is within a limited-access website
https://link.springer.com/content/pdf/10.1007%2Fs00122-006-0292-4
Last checked: 10/05/2017
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Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Hirata, Mariko;
Cai, Hongwei;
Inoue, Maiko;
Yuyama, Nana;
Miura, Yuichi;
Komatsu, Toshinori;
Takamizo, Tadashi;
Fujimori, Masahiro |
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| Author Affiliation: | Hirata, Cai, Inoue, Yuyama, and Miura: Forage Crop Research Institute, Japan Grassland Agriculture and Forage Seed Association, Nasushiobara; Komatsu, Takamizo, and Fujimori: National Institute of Livestock and Grassland Science, Nishinasuno; Fujimori: Yamanashi Prefectural Dairy Experiment Station, Hokuto, Japan |
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| Title: | Development of simple sequence repeat (SSR) markers and construction of an SSR-based linkage map in Italian ryegrass (Lolium multiflorum Lam.) |
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| Source: | TAG: Theoretical and Applied Genetics. Vol. 113, No. 2, July 2006, p. 270-279. |
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| Publishing Information: | Berlin, Germany: Springer |
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| # of Pages: | 10 |
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| Related Web URL: | http://link.springer.com/article/10.1007%2Fs00122-006-0292-4
Last checked: 03/27/2012
Notes: Abstract only |
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| Keywords: | TIC Keywords: Chromosome analysis; Lolium multiflorum; Simple sequence repeats
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| Abstract/Contents: | "In order to develop simple sequence repeat (SSR) markers in Italian ryegrass, we constructed a genomic library enriched for (CA)n-containing SSR repeats. A total of 1,544 clones were sequenced, of which 1,044 (67.6%) contained SSR motifs, and 395 unique clones were chosen for primer design. Three hundred and fifty-seven of these clones amplified products of the expected size in both parents of a two-way pseudo-testcross F1 mapping population, and 260 primer pairs detected genetic polymorphism in the F1 population. Genetic loci detected by a total of 218 primer pairs were assigned to locations on seven linkage groups, representing the seven chromosomes of the haploid Italian ryegrass karyotype. The SSR markers covered 887.8 cM of the female map and 795.8 cM of the male map. The average distance between two flanking SSR markers was 3.2 cM. The SSR markers developed in this study will be useful in cultivar discrimination, linkage analysis, and marker-assisted selection of Italian ryegrass and closely related species." |
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| Language: | English |
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| References: | 52 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hirata, M., H. Cai, M. Inoue, N. Yuyama, Y. Miura, T. Komatsu, et al. 2006. Development of simple sequence repeat (SSR) markers and construction of an SSR-based linkage map in Italian ryegrass (Lolium multiflorum Lam.). Theor. Appl. Genet. 113(2):p. 270-279. |
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| DOI: 10.1007/s00122-006-0292-4 |
| Web URL(s):
https://link.springer.com/article/10.1007/s00122-006-0292-4/fulltext.html
Last checked: 10/05/2017
Access conditions: Item is within a limited-access website
https://link.springer.com/content/pdf/10.1007%2Fs00122-006-0292-4
Last checked: 10/05/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2203373 |
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