Full TGIF Record # 200487
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DOI:10.1007/s00122-005-0119-8
Web URL(s):https://link.springer.com/article/10.1007/s00122-005-0119-8/fulltext.html
    Last checked: 10/05/2017
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https://link.springer.com/content/pdf/10.1007/s00122-005-0119-8
    Last checked: 10/05/2017
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Publication Type:
i
Refereed
Author(s):Shinozuka, H.; Hisano, H.; Ponting, R. C.; Cogan, N. O. I.; Jones, E. S.; Forster, J. W.; Yamada, T.
Author Affiliation:Shinozuka, Hisano and Yamada: Field Science Center for Northern Biosphere, Hokkaido University, Sapporo, Japan; Ponting, Cogan, Jones and Forster: Primary Industries Research Victoria, Plant Biotechnology Centre and Molecular Plant Breeding Cooperative Research Centre, La Trobe University, Bundoora, Victoria, Australia; Jones: Crop Genetics Research and Development, Pioneer Hi-Bred International, Johnston, IO [IA]; Yamada: National Agricultural Research Center for Hokkaido Region, National Agriculture and Bio-oriented Research Organization, Sapporo, Japan
Title:Molecular cloning and genetic mapping of perennial ryegrass casein protein kinase 2 α-subunit genes
Source:TAG: Theoretical and Applied Genetics. Vol. 112, No. 1, December 2005, p. 167-177.
Publishing Information:Berlin, Germany: Springer
# of Pages:11
Related Web URL:http://link.springer.com/article/10.1007/s00122-005-0119-8
    Last checked: 04/01/2013
    Notes: Abstract only
Keywords:TIC Keywords: Casein amino acids; Cold resistance; Flowering; Gene expression; Hybridization; Lolium perenne; Phenotypic correlation; Reproductive fertility; Single nucleotide polymorphism
Abstract/Contents:"The α-subunit of the casein protein kinase CK2 has been implicated in both light-regulated and circadian rhythm-controlled plant gene expression, including control of the flowering time. Two putative CK2α genes of perennial ryegrass (Lolium perenne L.) have been obtained from a cDNA library constructed with mRNA isolated from cold-acclimated crown tissue. The genomic organisation [organization] of the two genes was determined by Southern hybridisation [hybridization] analysis. Primer designs to the Lpck2a-1 and Lpck2a-2 cDNA sequences permitted the amplification of genomic products containing large intron sequences. Amplicon sequence analysis detected single nucleotide polymorphisms (SNPs) within the p150/112 reference mapping population. Validated SNPs, within diagnostic restriction enzyme sites, were used to design cleaved amplified polymorphic sequence (CAPS) assays. The Lpck2a-1 CAPS marker was assigned to perennial ryegrass linkage group (LG) 4 and the Lpck2a-2 CAPS marker was assigned to LG2. The location of the Lpck2a-1 gene locus supports the previous conclusion of conserved synteny between perennial ryegrass LG4, the Triticeae homoeologous group 5L chromosomes and the corresponding segment of rice chromosome 3. Allelic variation at the Lpck2a-1 and Lpck2a-2 gene loci was correlated with phenotypic variation for heading date and winter survival, respectively. SNP polymorphism may be used for the further study of the role of CK2α genes in the initiation of reproductive development and winter hardiness in grasses."
Language:English
References:44
Note:Pictures, b/w
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Shinozuka, H., H. Hisano, R. C. Ponting, N. O. I. Cogan, E. S. Jones, J. W. Forster, et al. 2005. Molecular cloning and genetic mapping of perennial ryegrass casein protein kinase 2 α-subunit genes. Theor. Appl. Genet. 112(1):p. 167-177.
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DOI: 10.1007/s00122-005-0119-8
Web URL(s):
https://link.springer.com/article/10.1007/s00122-005-0119-8/fulltext.html
    Last checked: 10/05/2017
    Access conditions: Item is within a limited-access website
https://link.springer.com/content/pdf/10.1007/s00122-005-0119-8
    Last checked: 10/05/2017
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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