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DOI: | 10.1007/s11032-007-9083-0 |
Web URL(s): | https://link.springer.com/article/10.1007%2Fs11032-007-9083-0 Last checked: 10/05/2017 |
Publication Type:
| Refereed |
Author(s): | Stein, Juliana;
Pessino, Silvina C.;
MartĂnez, Eric J.;
Rodriguez, MarĂa PĂa;
Siena, Lorena A.;
Quarin, Camilo L.;
Ortiz, Juan Pablo Amelio |
Author Affiliation: | Stein, Pessino, Rodriguez, Siena, and Ortiz: Laboratorio de BiologĂa Molecular, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla; MartĂnez, Quarin, and Ortiz: Instituto de Botánica del Nordeste (IBONE)- CONICET, Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Argentina |
Title: | A genetic map of tetraploid Paspalum notatum FlĂĽgge (bahiagrass) based on single-dose molecular markers |
Source: | Molecular Breeding. Vol. 20, No. 2, September 2007, p. 153-166. |
Publishing Information: | The Netherlands: Kluwer Academic Publishers |
# of Pages: | 14 |
Keywords: | TIC Keywords: Gene mapping; Genetic linkage; Genetic markers; Paspalum notatum; Tetraploidy
|
Abstract/Contents: | "Paspalum notatum Flu^D"gge is a warm-season forage grass with mainly diploid (2n = 20) and autotetraploid (2n = 40) representatives. Diploid races reproduce sexually and require crosspollination due to a self-incompatible mating system, while autotetraploids reproduce by aposporous apomixis. The objectives of this work were to develop a genetic linkage map of Paspalum notatum Flu^D"gge at the tetraploid level, identify the linkage/s group/s associated with apomixis and carry out a general characterization of its mode of inheritance. A pseudo test-cross F1 family of 113 individuals segregating for the mode of reproduction was obtained by crossing a synthetic completely sexual tetraploid plant (Q4188) as female parent with a natural aposporous individual (Q4117) as pollen donor. Map construction was based on single-dose markers (SDAFs) segregating from both parents. Two linkage maps (female and male) were constructed. Within each map, homologous groups were assembled by detecting repulsion-phase linked SDAFs. Putative Q4188 and Q4117 homolog groups were identified by mapping shared single dose markers (BSDF). The Q4188 map consisted of 263 markers distributed on 26 co-segregation groups over a total genetic distance of 1.590.6 cM, while the Q4117 map contained 216 loci dispersed on 39 co-segregation groups along 2.265.7 cM, giving an estimated genome coverage of 88% and 83%, respectively. Seven and 12 putative homologous chromosomes were detected within Q4188 and Q4117 maps, respectively. Afterward, ten female and male homologous chromosomes were identified by mapping BSDFs. In the Q4117 map, a single linkage group was associated with apospory. It was characterized by restriction in recombination and preferential chromosome pairing. A BPSD marker mapping within this group allowed the detection of the female homolog and the putative four male groups of the set carrying apospory." |
Language: | English |
References: | 41 |
Note: | Figures Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Stein, J., S. C. Pessino, E. J. MartĂnez, M. P. Rodriguez, L. A. Siena, C. L. Quarin, et al. 2007. A genetic map of tetraploid Paspalum notatum FlĂĽgge (bahiagrass) based on single-dose molecular markers. Molecular Breeding. 20(2):p. 153-166. |
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| DOI: 10.1007/s11032-007-9083-0 |
| Web URL(s): https://link.springer.com/article/10.1007%2Fs11032-007-9083-0 Last checked: 10/05/2017 |
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