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| Publication Type:
| Refereed |
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| Author(s): | Johnson-Flanagan, Anne M.;
Huiwen, Zhong;
Thiagarajah, Mohan R.;
Hargurdeep, S. Saini |
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| Author Affiliation: | Department of Plant Science, University of Alberta, Edmonton, Alberta, Canada |
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| Title: | Role of abscisic acid in the induction of freezing tolerance in Brassica napus suspension-cultured cells |
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| Source: | Plant Physiology. Vol. 95, No. 4, April 1991, p. 1044-1048. |
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| Publishing Information: | Lancaster, PA: American Society of Plant Physiologists |
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| Keywords: | TIC Keywords: Brassica napus; Abscisic acid; Cold resistance
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| Abstract/Contents: | "Brassica napus suspension-cultured cells could be hardened in 6 days at 25 degrees C by the addition of mefluidide or ABA to the culture medium. Cells treated with mefluidide (10 milligrams per liter) or ABA (50 micromolar) attained an LT50 of -17.5 degrees C or -18 degrees C, respectively, while the LT50 for the comparable nonhardened control (sucrose) was -10 degrees C. The increased freezing tolerance of mefluidide-treated cells was paralleled by a 4- to 23-fold increase in ABA, as measured by gas-liquid chromatography using electorn capture detection. Application of 1 milligram per liter of fluridone, an inhibitor of abscisic acid biosynthesis, prevented the mefluidide-induced increase in freezing tolerance and the accumulation of ABA. Both these inhibitory effects of fluridone were overridden by 50 micromolar ABA in the culture medium. On the basis of these results, we concluded that increased ABA levels are important for the induction of freezing tolerance in suspension-cultured cells." |
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| Language: | English |
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| References: | 19 |
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| Note: | Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Johnson-Flanagan, A. M., Z. Huiwen, M. R. Thiagarajah, and S. S. Hargurdeep. 1991. Role of abscisic acid in the induction of freezing tolerance in Brassica napus suspension-cultured cells. Plant Physiol. 95(4):p. 1044-1048. |
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