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| DOI: | 10.1094/PHYTO-102-7-S4.1 |
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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-102-7-S4.1#page=7
Last checked: 08/09/2012
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Aynardi, B. A.;
Jimenez-Gasco, M. M.;
Uddin, W. |
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| Author Affiliation: | The Pennsylvania State University, University Park, PA |
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| Title: | Molecular detection and host adaptation of Sclerotinia homoeocarpa, the causal agent of dollar spot of turfgrass |
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| Meeting Info.: | Providence, Rhode Island: August 4-8, 2012 |
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| Source: | Phytopathology. Vol. 102, No. 7S, July 2012, p. S4.7-S4.8. |
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| Publishing Information: | St. Paul, Minnesota: American Phytopathological Society |
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| # of Pages: | 2 |
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| Keywords: | TIC Keywords: Disease development; Disease evaluation; Disease identification; Dollar spot; Maintenance planning; Sclerotinia homoeocarpa
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| Abstract/Contents: | "Dollar spot of turfgrass, caused by Sclerotinia homoeocarpa, affects cool and warm-season grasses throughout North America. The objective of this project was to develop a molecular method for the detection of pathogen DNA. Sequencing of the Internal Transcribed Spacer (ITS) region of the rDNA and comparisons of numerous dollar spot isolates collected throughout North America in the past three decades allowed for the design of species-specific primers that are able to amplify only DNA of the pathogen of interest. Specificity of the two newly designed primers was confirmed through testing its closest relatives in the Rutstroemiaceae and other turfgrass pathogens for amplification of DNA, which did not occur in either case. The implementation of these primers for the quantification of inoculum levels in the thatch/soil interface to predict the onset of disease will allow for proper fungicide application timing in early spray programs. Additionally, phylogenetic analysis of the ITS region showed that two isolates concurrently collected from a bentgrass/Poa putting green and a bermudagrass fairway on the same golf course were genetically distinct. The maximum likelihood analysis indicated that the bermudagrass isolate grouped with other isolates from bermudagrass previously collected in Florida. This example provides direct evidence that genetically diverse populations of S. homoeocarpa host adapted for cool and warm-season grasses coexist in the same locality." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Aynardi, B. A., M. M. Jimenez-Gasco, and W. Uddin. 2012. Molecular detection and host adaptation of Sclerotinia homoeocarpa, the causal agent of dollar spot of turfgrass. Phytopathology. 102(7S):p. S4.7-S4.8. |
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| DOI: 10.1094/PHYTO-102-7-S4.1 |
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-102-7-S4.1#page=7
Last checked: 08/09/2012
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b2219736a |
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