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Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1991Articles/phyto81n06_701.pdf#page=5 Last checked: 10/15/2015 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Refereed |
Content Type: | Abstract or Summary Only |
Author(s): | Turner, John T.;
Lampel, Jay S.;
Sundin, George W. |
Author Affiliation: | Crop Genetics International, Hanover, Maryland |
Title: | Deletion of engineered DNA sequences from a recombinant strain of Clavibacter XYLI Subsp. Cynodontis. |
Source: | Phytopathology. Vol. 81, No. 6, June 1991, p. 705. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
# of Pages: | 1 |
Keywords: | TIC Keywords: DNA; Genetics; Genetic transformation; Techniques; Cynodon
|
Abstract/Contents: | Segregation of chromosomally inserted DNA sequences from an engineered strain of Clavibacter xyli subsp. synodontis was studied both in vitro and in planta. Populations of cells lacking the gene encoding the delta-endotoxin protein from Bacillus thuringiensis subsp. kurstaki and genes conferring antibiotic resistance were detected in bermudagrass and in shake flasks originally inoculated with pure cultures of a recombinant strain. In both cases the segregant population increased over time to result in a sigmoidal curve in which the segregant population approached 100 percent. Segregant cells were shown to have a higher growth rate than recombinant cells in vitro, thus resembling the wild-type. The environmental consequences of the extinction of engineered genes will be discussed. |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Turner, J. T., J. S. Lampel, and G. W. Sundin. 1991. Deletion of engineered DNA sequences from a recombinant strain of Clavibacter XYLI Subsp. Cynodontis.. Phytopathology. 81(6):p. 705. |
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| Web URL(s): http://www.apsnet.org/publications/phytopathology/backissues/Documents/1991Articles/phyto81n06_701.pdf#page=5 Last checked: 10/15/2015 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: SB 599 .P48 |
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