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| DOI: | 10.1094/PHP-2012-1024-01-RS |
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| Web URL(s): | https://www.plantmanagementnetwork.org/sub/php/research/2012/ryegrass/ryegrass.pdf
Last checked: 11/11/2016
Requires: PDF Reader
https://www.plantmanagementnetwork.org/sub/php/research/2012/ryegrass/
Last checked: 11/11/2016 |
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| Publication Type:
| Refereed |
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| Author(s): | Meng, Shawn;
Alderman, Steve;
Fraley, Cindy;
Ludy, Robin;
Sun, Fengjie;
Osterbauer, Nancy |
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| Author Affiliation: | Meng, Fraley, Ludy and Osterbauer: Commodity Inspection Division, Oregon Department of Agriculture, Salem, OR; Alderman: USDA-ARS National Forage Seed Production Research Center, Corvallis, OR; Sun: School of Science and Technology, Georgia Gwinnett College, Lawrenceville, GA; |
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| Title: | Identification of Anguina funesta from annual ryegrass seed lots in Oregon |
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| Section: | Plant health research
Other records with the "Plant health research" Section
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| Source: | Plant Health Progress. October 24 2012, p. [1-11]. |
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| Publishing Information: | St. Paul, Minnesota: Plant Management Network |
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| # of Pages: | 11 |
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| Keywords: | TIC Keywords: Anguina; DNA amplification; Enzymes; Evaluative methods; Galls; Lolium multiflorum; Morphological evaluation; Nematoda; Polymerase chain reaction; Rathayibacter toxicus; Restriction fragment length polymorphism; Seed testing; Species identification; Variety trials
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| Abstract/Contents: | "In 2010, seed galls containing Anguina sp. were isolated from 14 annual ryegrass (Lolium multiflorum) seed lots submitted for phytosanitary testing. To identify the species present, the ITS1 region of the ribosomal DNA of the nematodes from the seed lots was analyzed using a PCR-RFLP method. All nematodes produced a single 540-bp DNA amplicon, which was digested with three restriction enzymes, HaeIII, HinfI, and TaqI. The resulting RFLP patterns matched those of A. funesta. To confirm these results, 525 bp of the DNA amplicon was analyzed by DNA sequencing and BLAST analysis, which verified the sequence was identical to A. funesta (Genbank Accession nos. AF363095, AF363096, and AF363104). Because of the association of A. funesta with Rathayibacter toxicus, a second PCR test was conducted to determine if the bacterium was present in the seed lots. A 200-bp DNA amplicon was amplified from two seed galls, sequenced, and subjected to BLAST analysis. Analysis of the entire DNA sequence failed to identify the bacterium present, although testing by USDA-APHIS verified the bacterium was not R. toxicus. This is the first report of A. funesta in the US; R. toxicus was not found with this detection." |
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| Language: | English |
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| References: | 23 |
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| Note: | Pictures, b/w
Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Meng, S., S. Alderman, C. Fraley, R. Ludy, F Sun, and N. Osterbauer. 2012. Identification of Anguina funesta from annual ryegrass seed lots in Oregon. Plant Health Progress. p. [1-11]. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=212702 |
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| DOI: 10.1094/PHP-2012-1024-01-RS |
| Web URL(s):
https://www.plantmanagementnetwork.org/sub/php/research/2012/ryegrass/ryegrass.pdf
Last checked: 11/11/2016
Requires: PDF Reader
https://www.plantmanagementnetwork.org/sub/php/research/2012/ryegrass/
Last checked: 11/11/2016 |
| 
MSU catalog number: b4850509 |
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