Full TGIF Record # 215181
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Web URL(s):http://img.kisti.re.kr/soc_img/society//tsk/JDHHBF/2009/v23n2/JDHHBF_2009_v23n2_307.pdf
    Last checked: 02/11/2013
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Publication Type:
i
Refereed
Author(s):Jang, Duk-Hwan; Jung, Seung-Ho
Author Affiliation:Korea Turfgrass Research Institute, Seongnam, Korea
Title:Development of SCAR markers in creeping bentgrass (Agrostis palustris Huds.) cultivars
Source:Korean Journal of Turfgrass Science. Vol. 23, No. 2, 2009, p. 307-315.
Publishing Information:Korea: Turfgrass Society of Korea
# of Pages:9
Keywords:TIC Keywords: Agrostis stolonifera; Cultivar evaluation; Cultivar identification; Genetic markers; Golf greens; Polymerase chain reaction; Random amplified polymorphic DNA markers; Sequence-characterized amplified regions
Abstract/Contents:"Creeping bentgrass (Agrostis palustrics [palustris] Huds.) is cool season turfgrass that is used for putting green in golf course. Creeping bentgrass cultivars are difficult to distinguish with the same species because of similar morphological characters and low level of genetic diversity. The SCAR markers using the specific DNA can be useful for differentiating between creeping bentgrass cultivars. Five RAPD primers were used for specific band detection among creeping bentgrass cultivars, penncross, penn A-4, crenshaw, L-93, CY-2, T-1. The pairs of SCAR primers for six cultivars were designed by the specific sequences of the bands that amplified by RAPD. Three of the six SCAR primers could not make the use as SCAR primers because the specific false bands were detected in all cultivars. The remaining pairs of SCAR primer, CY850F/R, T700F/R, L2900F/R, amplified the specific band at expected size for three cultivars, CY-2, T-1, L-93, respectively. The CY850F/R primer amplified a band of 850bp in CY-2 cultivar, the T700F/R primer amplified a band of 700bp in T-1 cultivar, and the L2900F/R primer amplified a band of 2.9kb in L-93 cultivar. In this study we developed the SCAR markers to identify and distinguish the inerseeded creeping bentgrass cultivars in a golf course green."
Language:Korean
References:22
Note:Abstract also appears in English
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Jang, D.-H., and S.-H. Jung. 2009. Development of SCAR markers in creeping bentgrass (Agrostis palustris Huds.) cultivars. (In Korean) Korean Journal of Turfgrass Science. 23(2):p. 307-315.
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Web URL(s):
http://img.kisti.re.kr/soc_img/society//tsk/JDHHBF/2009/v23n2/JDHHBF_2009_v23n2_307.pdf
    Last checked: 02/11/2013
    Requires: PDF Reader
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