Full TGIF Record # 215375
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Web URL(s):http://www.cropj.com/Hassena_3_6_2009_322_328.pdf
    Last checked: 02/15/2013
    Requires: PDF Reader
Publication Type:
i
Refereed
Author(s):Ahmed, Salim; Nabi, Md. Zinnatun; Alam, Md. Maksudul; Islam, Md. Sazzadul; Samira, Rozalynne; Moosa, Mahdi M; Khan, Haseena
Author Affiliation:Ahmed and Moosa: Department of Genetic Engineering and Biotechnology; Alam, Islam, Samira, and Khan: Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh; Nabi: Department of Biochemistry and Medical Genetics, University of Manitoba, Canada
Title:A computational and experimental approach for developing jute ESTs from genomic clones
Source:Australian Journal of Crop Science. Vol. 3, No. 6, November 2009, p. 322-328.
# of Pages:7
Publishing Information:Australia: Southern Cross Publishing Group
Related Web URL:http://www.cropj.com/november2009.html
    Last checked: 03/11/2013
    Notes: Document is within a single large file; Abstract only
Keywords:TIC Keywords: Evaluative methods; Expressed sequence tag; Genetic analysis; Reverse transcriptase polymerase chain reaction
Abstract/Contents:"Gene identification is a leading and essential step in understanding the genome of an organism or for manipulation of genes necessary for incorporating any novel characteristics. However, current technologies and resources are far from shedding light on the genome of organisms like jute for which the whole genome sequence is yet to be determined. An alternative approach for finding genes in such genomes is to find and characterize specific genes, especially those which are differentially expressed under certain conditions. Though expressed sequence tags (ESTs) are widely used for this purpose, generation and characterization of ESTs are not straight forward in many cases. In this situation the available genomic clones can be used to identify and characterize ESTs with the combination of accessible bioinformatics tools such as gene prediction software, NCBI web portal etc and experimental approaches such as RT-PCR followed by sequencing. A number of ESTs for jute have been developed from a previously constructed jute genomic library enriched for simple sequence repeats and also by using the technique of differential display. Some of these ESTs were found to be interesting in terms of their agronomic properties."
Language:English
References:24
Note:Pictures, b/w
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Ahmed, S., M. Z. Nabi, M. M. Alam, M. S. Islam, R. Samira, M. M Moosa, et al. 2009. A computational and experimental approach for developing jute ESTs from genomic clones. Australian Journal of Crop Science. 3(6):p. 322-328.
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    Last checked: 02/15/2013
    Requires: PDF Reader
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