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| Web URL(s): | http://www.jstor.org/stable/pdf/23433409.pdf
Last checked: 01/29/2016
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Barker, Reed E.;
Barker, Judy |
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| Author Affiliation: | Grass Genomic Testing, Inc., Salem, OR |
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| Title: | Intolerance with annual-perennial ryegrass testing using DNA: Results of the STRF study across labroatories |
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| Section: | Abstracts
Other records with the "Abstracts" Section
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| Meeting Info.: | Des Moines, Iowa: May 20-24, 2012 |
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| Source: | Seed Technology. Vol. 34, No. 2, 2012, p. 286-287. |
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| Publishing Information: | Ithaca, New York: Association of Official Seed Analysts and Society of Commercial Seed Technologists |
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| # of Pages: | 2 |
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| Keywords: | TIC Keywords: Control methods; Lolium multiflorum; Lolium perenne; Testing
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| Abstract/Contents: | "Seed analysts and seed control agencies have become intolerant of ryegrass (Lolium spp.) tests being out of tolerance. Seedling root fluorescence (SRF) has been used to distinguish annual (L. Multiflorum Lam.) from perennial (L. Perenne L.) ryegrass for years. For some samples, however, this test has proven to be highly variable across laboratories doing the testing, with test results being out of tolerance and resulting in stop sales. A ryegrass allelic discrimination (RAD) test that examines variants in DNA sequences closely associated with plant growth types has been developed. When examined across three laboratories, there were differences among the laboratories for some samples when the RAD test was coupled with SRF. Statistical analyses, however, found that when variation associated with SRF alone was removed, there were no significant differences among laboratories in RAD test results. Tolerance tables are based on normal sample-to-sample variation (sampling error) and on variation associated with conducting the test itself (experimental error). To develop accurate tolerance tables for the RAD test, a grant from the Seed Technology Research Foundation (STRF) was provided in order to examine aspects of experimental error. The objective of the STRF study was to examine the actual RAD reaction values and final decisions of growth type prediction measured in four laboratories. Our attempt was to control all other sources of experimental variation in the study. Both among and within the laboratory sources of RAD reaction variation were measured by including "blind" duplicate samples. Reactions were determined on a seedling by seedling basis, rather than by seed lot, because the RAD test is based on genetic determination on an individual seed/plant basis. Variation among duplicate samples within laboratories was trivial and not significant. Only one plant in each of two laboratories was classified differently than its duplicate. Preliminary data analysis indicated that deviations from expected results among laboratories were also insignificant, indicating that standard tolerance tables based on sampling error alone may be sufficient for the RAD test. The RAD test relies on a SRF prescreening to reduce costs. Because of the apparent large experimental variation associated with SRF, however, we suggest that updated tolerance tables based on actual experimental evidence for the SRF test be developed and those tables also be used for the RAD test." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Barker, R. E., and J. Barker. 2012. Intolerance with annual-perennial ryegrass testing using DNA: Results of the STRF study across labroatories. Seed Technology. 34(2):p. 286-287. |
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| Web URL(s):
http://www.jstor.org/stable/pdf/23433409.pdf
Last checked: 01/29/2016
Requires: PDF Reader
Access conditions: Item is within a limited-access website
Notes: Item is within a single large file |
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MSU catalog number: b3628131 |
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