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| DOI: | 10.1111/wre.12002 |
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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1111/wre.12002/pdf
Last checked: 02/25/2013
Requires: PDF Reader
Access conditions: Item is within a limited access website
http://onlinelibrary.wiley.com/doi/10.1111/wre.12002/full
Last checked: 02/25/2013
Access conditions: Item is within a limited access website |
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| Publication Type:
| Refereed |
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| Author(s): | Alarcón-Reverte, R.;
Hanley, S.;
Kaundun, S. S.;
Karp, A.;
Moss, S. R. |
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| Author Affiliation: | Alarcón-Reverte: Department of Plant Sciences, University of California, Davis, CA, USA; Alaracón-Reverte, Hanley, Karp, and Moss: Rothamsted Research, Harpenden, Herts; Kaundun: Syngenta, Jealott's Hill International Research Centre, Bracknell, UK |
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| Title: | A SNaPshot assay for the rapid and simple detection of known point mutations conferring resistance to ACCase-inhibiting herbicides in Lolium spp. |
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| Source: | Weed Research. Vol. 53, No. 1, February 2013, p. 12-20. |
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| Publishing Information: | Oxford, United Kingdom: Blackwell Scientific Publications |
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| # of Pages: | 9 |
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| Related Web URL: | http://onlinelibrary.wiley.com/doi/10.1111/wre.12002/abstract
Last checked: 03/11/2013
Notes: Abstract only |
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| Keywords: | TIC Keywords: Control methods; Genetic analysis; Genotyping; Herbicide efficacy; Herbicide resistance; Lolium multiflorum; Research priorities; Weed control
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| Abstract/Contents: | "Evolution of resistance to herbicides in weeds is becoming an increasing problem worldwide. To develop effective strategies for weed control, a thorough knowledge of the basis of resistance is required. Although non-target-site-based resistance is widespread, target site resistance, often caused by a single nucleotide change in the gene encoding the target enzyme, is also a common factor affecting the efficacies of key herbicides. Therefore, fast and relatively simple high-throughput screening methods to detect target site resistance mutations will represent important tools for monitoring the distribution and evolution of resistant alleles within weed populations. Here, we present a simple and quick method that can be used to simultaneously screen for up to 10 mutations from several target site resistance-associated codons in a single reaction. As a proof of concept, this SNaPshot multiplex method was successfully applied to the genotyping of nine variable nucleotide positions in the CT domain of the chloroplastic ACCase gene from Lolium multiflorum plants from 54 populations. A total of 10 nucleotide substitutions at seven of these nine positions (namely codons 1781, 1999, 2027, 2041 2078, 2088 and 2096) are known to confer resistance to ACCase-inhibiting herbicides. This assay has several advantages when compared with other methods currently in use in weed science. It can discriminate between different nucleotide changes at a single locus, as well as screening for SNPs from different target sites by pooling multiple PCR products within a single reaction. The method is scalable, allowing reactions to be carried out in either 96- or 384-well plate formats, thus reducing work time and cost." |
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| Language: | English |
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| References: | 36 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Alarcón-Reverte, R., S. Hanley, S. S. Kaundun, A. Karp, and S. R. Moss. 2013. A SNaPshot assay for the rapid and simple detection of known point mutations conferring resistance to ACCase-inhibiting herbicides in Lolium spp.. Weed Res. 53(1):p. 12-20. |
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| DOI: 10.1111/wre.12002 |
| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1111/wre.12002/pdf
Last checked: 02/25/2013
Requires: PDF Reader
Access conditions: Item is within a limited access website
http://onlinelibrary.wiley.com/doi/10.1111/wre.12002/full
Last checked: 02/25/2013
Access conditions: Item is within a limited access website |
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MSU catalog number: b2221093 |
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