Full TGIF Record # 21689
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DOI:10.21273/HORTSCI.26.6.766C
Web URL(s):https://journals.ashs.org/hortsci/view/journals/hortsci/26/6/article-p766C.xml
    Last checked: 11/08/2019
    Requires: PDF Reader
Publication Type:
i
Refereed
Author(s):Moore, Ronald W.; Eskridge, K. M.; Read, P. F.; Riordan, T. P.
Author Affiliation:Departments of Horticulture and Biometry, University of Nebraska, Lincoln, NE
Title:Optimizing callus initiation using stolon nodal segments of Buffalograss NE84-609 and a response surface design
Source:HortScience. Vol. 26, No. 6, June 1991, p. [150] 766.
Publishing Information:St. Joseph, MI: American Society for Horticultural Science
Keywords:TIC Keywords: Bouteloua dactyloides; Calli; Stolons
Abstract/Contents:"The concept that greater callus mass will induce competence was investigated. The second most immature nodal segments were removed from heavily fertigated greenhouse grown plants. Shoots initiated from those nodes were only cut back to one-third their total length. They were subjected to the following treatments: (1) dicamba from 1mu M to 5mu M in increments of 1.0; (2) B5 medium salt concentrations from 1/3x to 5/3x in ingrements of 1/3; (3) sucrose levels from 2% to 10% in increments of 2; (4) casein hydrolysate from 0 to 200mg/l in increments of 50. The experiment consisted of twenty-five different treatment combinations in a central composite rotatable second order design. Explants were placed in continuous cool white flourescent light at 26C. Dicamba, B5 salts, and sucrose had significant effects on callus mass (p greater than or equal to .12), while casein hydrolysate had no notable effects on callus mass (p greater than or equal to .57). It was determined that optimum response occurred at 5/3x concentration of B5 salts, 10% sucrose, and 5.0mu M dicamba. White, compact calli were observed in treatment combinations that yielded callus fresh weights of two-hundred milligrams or higher."
Language:English
References:0
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Moore, R. W., K. M. Eskridge, P. F. Read, and T. P. Riordan. 1991. Optimizing callus initiation using stolon nodal segments of Buffalograss NE84-609 and a response surface design. HortScience. 26(6):p. [150] 766.
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DOI: 10.21273/HORTSCI.26.6.766C
Web URL(s):
https://journals.ashs.org/hortsci/view/journals/hortsci/26/6/article-p766C.xml
    Last checked: 11/08/2019
    Requires: PDF Reader
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MSU catalog number: SB 1 .H64
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