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Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1991Articles/phyto81n10_1133.pdf#page=12 Last checked: 10/15/2015 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Young, S. A.;
Bender, C. L.;
Park, S. K. |
Author Affiliation: | Department of Plant Pathology, Oklahoma State University, Stillwater |
Title: | Cloning and characterizations of coronatine genes in plasmid p418A from Pseudomonas syringae pv. glycinea 4180 |
Source: | Phytopathology. Vol. 81, No. 10, October 1991, p. 1145 #77. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
Keywords: | TIC Keywords: Pseudomonas syringae; Chromatography
|
Abstract/Contents: | "We have recently demonstrated that coronatine synthesis genes reside on a 90 kb plasmid (p4180A) in Pseudomonas syringae pv. glycinea PG4180. In the present study, a cosmid library of p4180A was constructed in pLAFR3. A 54 kb cosmid clone, designated pSAY10, restored coronatine production to two coronatine-defective mutants of PG4180, indicating that pSAY10 contains some of the genes required for coronatine synthesis. pSAY10 was subjected to saturation mutagenesis with Tn5 and mutants selected were conjugated into PG4180 and recombined into p4180A by marker-exchange. Organic acids were extrected from these mutants and analyzed by reverse-phase high performance liquid chromatography to determine the effect of various Tn5 insertions on coronatine synthesis. Mutations in two regions of pSAY10 (17.9 and 2.2 kb) completely blocked the synthesis of coronafacic acid and coronatine." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Young, S. A., C. L. Bender, and S. K. Park. 1991. Cloning and characterizations of coronatine genes in plasmid p418A from Pseudomonas syringae pv. glycinea 4180. Phytopathology. 81(10):p. 1145 #77. |
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| Web URL(s): http://www.apsnet.org/publications/phytopathology/backissues/Documents/1991Articles/phyto81n10_1133.pdf#page=12 Last checked: 10/15/2015 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: SB 599 .P48 |
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