Full TGIF Record # 219327
Item 1 of 1
DOI:10.1094/PHYTO-102-1-S1.1
Web URL(s):http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-102-1-S1.1
    Last checked: 04/25/2013
    Requires: PDF Reader
    Notes: Item is within a single large file
Publication Type:
i
Report
Content Type:Abstract or Summary only
Author(s):Ambrose, K. V.; Belanger, F. C.
Author Affiliation:Dept. of Plant Biology & Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ
Title:Counting genes and comprehending processes: How high-throughput sequencing is aiding in piecing together the grass-endophyte symbiosis puzzle
Section:2011 Norteastern Division meeting abstracts
Other records with the "2011 Norteastern Division meeting abstracts" Section
Meeting Info.:New Brunswick, New Jersey: October 12-14, 2011
Source:Phytopathology. Vol. 102, No. 1, Supplement 1 2012, p. S1.1.
Publishing Information:St. Paul, Minnesota: American Phytopathological Society
# of Pages:1
Keywords:TIC Keywords: Acremonium; Endophytes; Epichloe; Festuca rubra; Mutualism
Abstract/Contents:"Some grass species share a mutualistic relationship with fungal symbionts, the Neotyphodium and Epichloë endophytes. However, much research is needed to understand the dynamics of this interaction. How the plants gene expression profile is altered by the presence of the endophyte remains unclear. The goal of our research is to understand how grass species like the fine fescues (Festuca rubra) are able to maintain such a symbiosis, where the endophytes (E. festucae) nutrient requirement is met without the grass triggering its defense mechanism. Meanwhile, the grass gains from this interaction by way of resistance to insect and mammalian herbivores. Moreover, E. festucae infection of F. rubra confers fungal disease resistance to the host, an effect that has not been reported in other cool-season grass-endophyte interactions. We have utilized transcriptome sequencing to address fundamental aspects of this symbiosis. Our hypothesis is that differential gene expression is important for the maintenance of the interaction. We have used SAGE (Serial Analysis of Gene Expression) to obtain a quantitative comparison of the transcriptome of endophyte-free, and endophyte-infected plants. The > 54 million SAGE tags we obtained were supplemented with > 200 000 454 transcriptome sequences of the E. festucae endophyte strain isolated from its grass host plus the plant infected with that strain. The SAGE tags were mapped to a reference database consisting of our 454 transcriptome, and Festuca and Epichloë sequences from NCBI. Mapping of the SAGE tags yielded ~ 49 000 plant, and ~ 9 000 endophyte genes. Analysis of the data has revealed plant transcriptional changes in response to fungal endophyte infection. Differential gene expression patterns have been observed in categories related to plant growth, innate defense, abiotic stress-acclimation, and sugar storage. Ultimately, information obtained from this study will enrich our understanding of how symbiotic relationships in nature are maintained."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Ambrose, K. V., and F. C. Belanger. 2012. Counting genes and comprehending processes: How high-throughput sequencing is aiding in piecing together the grass-endophyte symbiosis puzzle. Phytopathology. 102(1):p. S1.1.
Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=219327
If there are problems with this record, send us feedback about record 219327.
Choices for finding the above item:
DOI: 10.1094/PHYTO-102-1-S1.1
Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-102-1-S1.1
    Last checked: 04/25/2013
    Requires: PDF Reader
    Notes: Item is within a single large file
Find Item @ MSU
MSU catalog number: b2219736a
Find from within TIC:
   Digitally in TIC by file name: phytp2012jannortheasterndiv
Request through your local library's inter-library loan service (bring or send a copy of this TGIF record)