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DOI: | 10.1094/PHYTO-103-6-S2.1 |
Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-103-6-S2.1#page=63 Last checked: 06/03/2013 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Hulvey, J.;
Bousquet, A.;
Sang, H.;
Jung, G. |
Author Affiliation: | University of Massachusetts, Amherst, MA |
Title: | Development of molecular diagnostic assays for fungicide resistance in an important turfgrass pathogen, Sclerotinia homoeocarpa |
Section: | 2013 APS-MSA Joint Meeting abstracts of presentations Other records with the "2013 APS-MSA Joint Meeting abstracts of presentations" Section
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Meeting Info.: | Austin, Texas: August 10-14, 2013 |
Source: | Phytopathology. Vol. 103, No. 6S, June supplement 2013, p. S2.63. |
Publishing Information: | St. Paul, Minnesota: American Phytopathological Society |
# of Pages: | 1 |
Keywords: | TIC Keywords: DMI fungicides; Dicarboximides; Dollar spot; Fungicide resistance; Methyl bromide; Sclerotinia homoeocarpa
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Trade Names: | Carbendazim |
Abstract/Contents: | "The dollar spot pathogen, Sclerotinia homoeocarpa, has demonstrated the ability to overcome the dicarboximide, Demethylation Inhibitor (DMI) and Methyl Benzimidazole Carbamate (MBC) fungicide classes in the United States. Reduced sensitivity and resistance to these three fungicide classes, which constitute important control options for dollar spot, indicates the need for an accurate and quick diagnostic assay for fungicide resistance in the dollar spot pathogen. By mining transcriptomic data of S. homoeocarpa, we have targeted the resistance determinants for these three fungicides, and designed PCR-based assays for the detection of fungicide resistance alleles and gene expression of fungicide resistance determinants in field isolates. Two techniques are being optimized for these assays: high-resolution DNA melting analysis (HR-DMA) and quantitative Real-Time PCR (qRT-PCR). Preliminary data indicate that HR-DMA and qRT-PCR can be used to detect resistance to all three classes. If implemented, these assays could help turfgrass managers to minimize costs and environmental consequences from overuse and misapplication of fungicides that prove ineffective at sites with resistance." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Hulvey, J., A. Bousquet, H. Sang, and G. Jung. 2013. Development of molecular diagnostic assays for fungicide resistance in an important turfgrass pathogen, Sclerotinia homoeocarpa. Phytopathology. 103(6S):p. S2.63. |
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| DOI: 10.1094/PHYTO-103-6-S2.1 |
| Web URL(s): http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-103-6-S2.1#page=63 Last checked: 06/03/2013 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: b2219736a |
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