Full TGIF Record # 220899
Item 1 of 1
DOI:10.1094/PHYTO-103-6-S2.1
Web URL(s):http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-103-6-S2.1#page=76
    Last checked: 06/03/2013
    Requires: PDF Reader
    Notes: Item is within a single large file
Publication Type:
i
Report
Content Type:Abstract or Summary only
Author(s):Lakshman, D. K.; Amaradasa, B. S.; Horvath, B.
Author Affiliation:Lakshman: USDA ARS, Beltsville, MD; Amaradasa: University of Nebraska-Lincoln, Lincoln, NE; Horvath: University of Tennessee, Knoxville, TN
Title:Development of SCAR markers and UP-PCR cross-hybridization method for specific detection of brown patch pathogens from infected turfgrasses
Section:2013 APS-MSA Joint Meeting abstracts of presentations
Other records with the "2013 APS-MSA Joint Meeting abstracts of presentations" Section
Meeting Info.:Austin, Texas: August 10-14, 2013
Source:Phytopathology. Vol. 103, No. 6S, June supplement 2013, p. S2.76.
Publishing Information:St. Paul, Minnesota: American Phytopathological Society
# of Pages:1
Keywords:TIC Keywords: Disease identification; Genetic markers; Patch diseases; Rhizoctonia solani; Thanatephorus cucumeris; Waitea circinata var. circinata; Waitea circinata var. zeae
Abstract/Contents:"Several species and hyphal anastomosis groups (AG) of Rhizoctonia solani (sensu lato) cause brown patch diseases of turfgrasses. Conventional methods of identification of Rhizoctonia pathogens are time consuming and often inaccurate. A rapid identification assay for Waitea circinata (anamorph: Rhizoctonia spp.) varieties zeae and circinata causing patch diseases was developed based on the universally primed PCR (UP-PCR) products cross-blot hybridization with non-radioactive probes. Isolates within a W. circinata variety cross-hybridized strongly while non-homologous isolates did not cross-hybridize or did so weakly. Closely related W. circinata varieties zeae and circinata were clearly distinguished using this assay. In addition, sequence-characterized amplified region (SCAR) markers were developed from UP-PCR products to identify turf pathogenic isolates of Thanatephorus cucumeris (anamorph: R. solani) AG 1-IB and AG 2-2IIIB. The developed SCAR markers could distinguish isolates of AG 1-IB or AG 2-2IIIB groups and did not amplify any product from genomic DNA of non-target isolates of Rhizoctonia. The specific primers were sensitive and unique enough to produce a PCR band from total DNA of diseased turfgrasses infected with either AG 1-IB or AG 2-2IIIB."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lakshman, D. K., B. S. Amaradasa, and B. Horvath. 2013. Development of SCAR markers and UP-PCR cross-hybridization method for specific detection of brown patch pathogens from infected turfgrasses. Phytopathology. 103(6S):p. S2.76.
Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=220899
If there are problems with this record, send us feedback about record 220899.
Choices for finding the above item:
DOI: 10.1094/PHYTO-103-6-S2.1
Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-103-6-S2.1#page=76
    Last checked: 06/03/2013
    Requires: PDF Reader
    Notes: Item is within a single large file
Find Item @ MSU
MSU catalog number: b2219736a
Find from within TIC:
   Digitally in TIC by file name: phytp2013junpres
Request through your local library's inter-library loan service (bring or send a copy of this TGIF record)