| |
| Web URL(s): | https://archive.lib.msu.edu/tic/its/articles/2013jou281.pdf
Last checked: 07/06/2015
Requires: PDF Reader |
|---|
| Access Restriction: | Certain MSU-hosted archive URLs may be restricted to legacy database members. |
|---|
| Publication Type:
| Refereed |
|---|
| Author(s): | Mulkey, Steven E.;
Zuleta, M. Carolina;
Van Esbroeck, George A.;
Lu, Huangjun;
Kenworthy, Kevin E.;
Milla-Lewis, Susana R. |
|---|
| Author Affiliation: | Mulkey, Zuleta, Van Esbroeck and Milla-Lewis: Dept. of Crop Science, North Carolina State Univ., Raleigh, NC; Lu: University of Florida, Everglades Research and Education Center, Belle Glade; Kenworthy: Agronomy Dept., University of Florida, Gainesville, FL |
|---|
| Title: | Genetic analysis of a St. Augustinegrass germplasm collection using AFLP markers |
|---|
| Section: | Genetics and breeding
Other records with the "Genetics and breeding" Section
|
|---|
| Meeting Info.: | Beijing, China: July 14-19, 2013 |
|---|
| Source: | International Turfgrass Society Research Journal. Vol. 12, 2013, p. 281-291. |
|---|
| Publishing Information: | Madison, Wisconsin: International Turfgrass Society |
|---|
| # of Pages: | 11 |
|---|
| Keywords: | TIC Keywords: Amplification fragment length polymorphisms; Cultivar evaluation; Germplasm; Molecular genetics; Ploidy; Shade resistance; Stenotaphrum secundatum
|
|---|
| Abstract/Contents: | "St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] is a widely used turfgrass in the southern United States, favored for its shade tolerance and lush ground cover. The University of Florida (UF) St. Augustinegrass germplasm collection represents the largest pool of variation for the species, yet little is known about its diversity at the molecular or cytological levels. This study was conducted to assess levels of molecular and cytological diversity in this collection, compare them to those present among publicly available germplasm, and evaluate possible associations between molecular diversity and ploidy level. Four ploidy levels were designated based on flow cytometry results with most accessions falling into the diploid and proximal tetraploid categories. Twelve AFLP primer pairs produced a total of 747 scorable bands. Unweighted pair group method with arithmetic averaging cluster (UPGMA) and principle coordinate analyses produced comparable groups of accessions that were based largely on ploidy level. Analysis of molecular variance indicated that both among and within ploidy levels were significant sources of variation. The number of observed bands largely increased with ploidy level, indicating greater levels of diversity among higher ploidy accessions. Among diploid accessions, one cluster was identified that was unrepresented by any cultivars or plant introductions. The UF St. Augustinegrass germplasm collection contains significant, untapped pools of diversity at diploid and polyploid levels and could be a great asset for cultivar development." |
|---|
| Language: | English |
|---|
| References: | 56 |
|---|
| Note: | Figures
Tables
Graphs |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Mulkey, S. E., M. C. Zuleta, G. A. Van Esbroeck, H. Lu, K. E. Kenworthy, and S. R. Milla-Lewis. 2013. Genetic analysis of a St. Augustinegrass germplasm collection using AFLP markers. Int. Turfgrass Soc. Res. J. 12:p. 281-291. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=223221 |
| If there are problems with this record, send us feedback about record 223221. |
| Choices for finding the above item: |
|---|
| Web URL(s):
https://archive.lib.msu.edu/tic/its/articles/2013jou281.pdf
Last checked: 07/06/2015
Requires: PDF Reader |
| 
MSU catalog number: b2394179 |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
