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| Web URL(s): | https://archive.lib.msu.edu/tic/its/articles/2013jou327.pdf
Last checked: 07/06/2015
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| Publication Type:
| Refereed |
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| Author(s): | Schwartz, Brian M.;
Harris-Shultz, Karen R.;
Contreras, Ryan N.;
Hans, Christian S.;
Hanna, Wayne W.;
Milla-Lewis, Susana R. |
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| Author Affiliation: | Schwartz and Hanna: Dept. of Crop & Soil Sciences, University of Georgia; Harris-Shultz: Crop Genetics and Breeding Research Unit, USDA-ARS, Tifton, GA; Contreras: Dept. of Horticulture, Oregon State University, Corvallis, OR; Hans: Dept. of Crop & Soil Sciences, University of Georgia, Athens, GA; Milla-Lewis: Dept. of Crop Sciences, North Carolina State University, Raleigh, NC |
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| Title: | Creation of artificial triploid and tetraploid centipedegrass using colchicine and breeding |
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| Section: | Genetics and breeding
Other records with the "Genetics and breeding" Section
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| Meeting Info.: | Beijing, China: July 14-19, 2013 |
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| Source: | International Turfgrass Society Research Journal. Vol. 12, 2013, p. 327-334. |
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| Publishing Information: | Madison, Wisconsin: International Turfgrass Society |
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| # of Pages: | 8 |
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| Keywords: | TIC Keywords: Colchicine; Epidermis; Eremochloa ophiuroides; Induced polyploidy; Low maintenance; Morphological evaluation; Sandy soils; Stomata
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| Cultivar Names: | TifBlair |
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| Abstract/Contents: | "Centipedegrass (Eremochloa ophiuroides) is a low maintenance, diploid (2n = 2x = 18) turfgrass species that is well adapted to sandy, acidic soils in the southern United States. Morphological variation and differential responses to distinct environmental conditions can be seen on an individual plant basis in this species, but phenotypic and genotypic variation is relatively small at the population level. Therefore, an effort was initiated in the spring of 2009 to induce polyploidy in 'TifBlair' centipedegrass using six colchicine seed treatments. Seedlings that germinated after exposure to these treatments were screened using flow cytometry for genome size changes. One putative tetraploid (63-9) and one cytochimera (63-8) were identified, both from the water/0.1% colchicine treatment. Stomata length of the colchicine induced putative tetraploid was 12% larger than that of TifBlair, but there was not a significant change in leaf stomata length in the cytochimera. The pollen diameter of the cytochimera was significantly larger than that of the putative tetraploid and TifBlair. Ploidy instability was discovered in the majority of 63-9 plant materials sampled in early 2011. Additionally, only five of the 580 seedlings derived from open-pollination of 63-9 in 2010 were triploid or tetraploid, further supporting that the L-II histogenic layer (germ line) of 63-9 was not stable at the time of fertilization. Results of flow cytometry analysis through 2011 of 63-8 have indicated that the chimeric state of this plant have remained constant, but all 19 seedlings derived from it have a 2C nuclear DNA content similar to that of a tetraploid. This information supports that DNA content of the L-II histogenic layer was doubled in 63-8, but that the L-I histogenic layer (epidermis) was still diploid. Evaluation of the turfgrass performance of these induced polyploids should be carried out to determine the value of this breeding procedure for improvement of centipedegrass." |
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| Language: | English |
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| References: | 37 |
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| Note: | Photomicrographs
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Schwartz, B. M., K. R. Harris-Shultz, R. N. Contreras, C. S. Hans, W. W. Hanna, and S. R. Milla-Lewis. 2013. Creation of artificial triploid and tetraploid centipedegrass using colchicine and breeding. Int. Turfgrass Soc. Res. J. 12:p. 327-334. |
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| Web URL(s):
https://archive.lib.msu.edu/tic/its/articles/2013jou327.pdf
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MSU catalog number: b2394179 |
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