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| Web URL(s): | https://archive.lib.msu.edu/tic/its/articles/2013jou805.pdf
Last checked: 07/06/2015
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| Publication Type:
| Refereed |
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| Author(s): | Wang, Ying;
Xu, Lixin;
Zeng, Huiming;
Tang, Wei;
Han, Liebao |
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| Author Affiliation: | Wang, Xu, Zeng and Han: Institute of Turfgrass Science, Beijing Forest University, Beijing; Tang: Yangtze University, Jingzhou, China |
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| Title: | Cloning and identifying the stress-inducible promoter Rd29A of Zoysia japonica Steud |
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| Section: | Short communications
Other records with the "Short communications" Section
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| Meeting Info.: | Beijing, China: July 14-19, 2013 |
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| Source: | International Turfgrass Society Research Journal. Vol. 12, 2013, p. 805-809. |
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| Publishing Information: | Madison, Wisconsin: International Turfgrass Society |
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| # of Pages: | 5 |
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| Keywords: | TIC Keywords: DNA; Gene expression; Molecular biology; Polymerase chain reaction; Stress physiology; Zoysia japonica
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| Abstract/Contents: | "Stress-inducible gene expression plays an important role in agricultural biotechnology and molecular biology. Cloning of stress-inducible promoter has not been reported in turfgrass. In this investigation, the stress-inducible promoter Rd29A was cloned from Zoysia japonica Steud via polymerase chain reaction (PCR). Sequence analysis of the cloned Rd29A DNA fragment (Genbank accession numberEU346948) indicates a 41.65% homology with the reported Arabidopsis Rd29A promoter sequence. To study the function of the Zoysia japonica Rd29A promoter, two plant expression vectors pBIG (35S-GFP) and pBIRG (Rd29AGFP) were constructed and used to transform the onion epidermal cells by particle bombardment. The Zoysia japonica Rd29A promoter regulated gfp gene expression was similar to the 35S promoter at room temperature. However, the GFP fluorescent intensity in pBIRG transformed cells was about two folds that of pBIG transformed cells at 4 °C. These results demonstrated that the Zoysia japonica Rd29A promoter was a stress-inducible promoter and it could be useful in stress biology of turfgrass. This is the first stress-inducible promoter isolated from a turfgrass species." |
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| Language: | English |
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| References: | 23 |
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| Note: | Pictures, b/w
Figures
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, Y., L. Xu, H. Zeng, W. Tang, and L. Han. 2013. Cloning and identifying the stress-inducible promoter Rd29A of Zoysia japonica Steud. Int. Turfgrass Soc. Res. J. 12:p. 805-809. |
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| Web URL(s):
https://archive.lib.msu.edu/tic/its/articles/2013jou805.pdf
Last checked: 07/06/2015
Requires: PDF Reader |
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MSU catalog number: b2394179 |
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