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| Web URL(s): | http://www.newss.org/proceedings/proceedings_2007.pdf#page=64
Last checked: 06/28/2013
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Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Sarkar, D.;
Bhowmik, P.;
Kwon, Y. I.;
Shetty, K. |
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| Author Affiliation: | Univ. of Massachusetts, Amherst |
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| Title: | Evaluation of proline-linked pentose phosphate pathway in creeping bentgrass |
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| Section: | Turfgrass and plant growth regulators
Other records with the "Turfgrass and plant growth regulators" Section
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| Meeting Info.: | Baltimore, Maryland: January 2-5, 2007 |
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| Source: | Proceedings of the Sixty-First Annual Meeting of the Northeastern Weed Science Society. Vol. 61, 2007, p. 46. |
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| Publishing Information: | Baltimore, Maryland: Northeastern Weed Science Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Cultivar profile; Evaluations; Pentose phosphate pathway; Proline
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| Abstract/Contents: | "Creeping bentgrass (Agrostis palustris Huds.) is an important cool-season turfgrass species in North America. It is used widely in putting greens, tees, bowling greens, and in grass tennis courts. Abiotic and biotic stresses are major hindrances for normal performance of the cool-season turfgrass. Under stress conditions plants produce reactive oxygen intermediates (singlet oxygen, etc.) within cells. Antioxidant defense systems scavenge reactive oxygen species and protect cells against oxidative stress injury. Plant phenolics play important role in this defense mechanism by providing UV protection, antifungal, antiviral, antibacterial, antifeedant and antimitotic activity. Phenolic antioxidants can either trap the free radicals or scavenge them through series of coupled antioxidant enzyme defense system. Proline plays a significant role in plant under stress condition by regulating redox and hydride ion-mediated stimulation of pentose phosphate pathway. Shetty (1997) proposed a role for proline-linked pentose phosphate pathway (PLPPP) in stimulating phenolic metabolites in plants. Proline is synthesized from glutamate through series of reduction reactions, and in this process pyrroline-5-carboxylate (P5C) and proline function as a redox couple and are known to be metabolic regulators. The proline, through reactions of proline dehydrogenase (PDH), can enter mitochondria and support oxidative phosphorylation (instead of NADH). The reduction of P5C provides NADP+, which is the co-factor for glucose-6-phosphate dehydrogenase (G6PDH), an enzyme that catalyzes the rate-limiting step of the pentose phosphate pathway. Proline-linked pentose phosphate pathway can stimulate both shikimate and phenylpropanoid pathways. Since, genetic heterogeneity in cross pollinated plant causes inconsistency in phenolic profiles, and related antioxidant enzyme response, screening of single seeded clonal lines is important to select an elite clonal line with superior phenolic profile and protective response. The objectives of this study are i) to understand the role of proline-linked pentose phosphate pathway for phenolic antioxidant production in creeping bentgrass, and ii) to evaluate overall antioxidant response system of creeping bentgrass. Single seeded creeping bentgrass plants (20 lines) were grown in 4.5 cm plastic pots with sand based soils and kept in growth chamber at 27 C (day/night), and 12-h photoperiod conditions. Turf was watered daily, mowed, and fertilized weekly. Shoots were collected and analyzed repeatedly for G6PDH, PDH, SDH (succinate dehydrogenase), CAT (catalase), GPX (guaiacol peroxidase), SOD (superoxide dismutase), DPPH (antioxidant activity), total phenolic, total protein, and chlorophyll. HPLC analyses of proline and phenolic profile were also undertaken. Results showed significant differences in PLPPP related enzymes such as G6PDH, and PDH among twenty creeping bentgrass lines. Similar trends were observed in case of antioxidant enzymes, like SOD, and GPX. Antioxidant activity (DPPH), and chlorophyll content also varied, but total phenolic, SDH, and CAT showed similar results in all twenty lines. Differences in activity of G6PDH, PDH, SOD, and GPX among the clonal lines provide clues to the significance of proline-linked pentose phosphate pathway in selected creeping bentgrass clonal lines." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Sarkar, D., P. Bhowmik, Y. I. Kwon, and K. Shetty. 2007. Evaluation of proline-linked pentose phosphate pathway in creeping bentgrass. Proc. Annu. Meet. Northeast. Weed Sci. Soc. 61:p. 46. |
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| Web URL(s):
http://www.newss.org/proceedings/proceedings_2007.pdf#page=64
Last checked: 06/28/2013
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 610 .N62 |
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