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| DOI: | 10.1080/07060661.2013.757422 |
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| Web URL(s): | http://www.tandfonline.com/doi/full/10.1080/07060661.2013.757422
Last checked: 07/03/2013
Notes: Item is within a single large file
http://www.tandfonline.com/doi/pdf/10.1080/07060661.2013.757422
Last checked: 07/03/2013
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Zheng, L.;
Shi, F.;
Hsiang, T. |
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| Author Affiliation: | Zheng: Key Lab of Plant Pathology of Hubei Proving, Huazhong Agricultural University, Wuhan, China; Shi and Hsiang: School of Environmental Sciences, University of Guelph, Guelph, ON, Canada |
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| Title: | Population structure of a Rhizoctonia solani population in a single field using ISSR markers |
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| Section: | Annual meeting, 2012
Other records with the "Annual meeting, 2012" Section
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| Source: | Canadian Journal of Plant Pathology. Vol. 35, No. 1, 2013, p. 131. |
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| Publishing Information: | [Canada]: Canadian Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Disease evaluation; Genetic distance; Genetic variability; Haplotypes; Inter simple sequence repeats; Rhizoctonia blight; Rhizoctonia solani
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| Abstract/Contents: | "Brown patch, caused by Rhizoctonia solani Kuhn, is an important disease of cool-season grasses, including bentgrasses, bluegrasses, fescues, and ryegrasses. The objective of this work was to examine the genetic diversity and population structure of R. solani in a single bentgrass ( Agrostis stolonifera ) field. In August 2011, 23 isolates of R. solani were obtained with a toothpick method from 17 patches of infected creeping bentgrass at the Guelph Turfgrass Institute, Guelph, Ontario. Genetic variation was analyzed by using five inter-simple sequence repeat (ISSR) primers. The five primers generated 34 polymorphic loci out of 50 band positions (68.0% polymorphism). The size of amplified bands ranged from 400 to 2000 bp. Cluster analysis of the resulting data was calculated with the unweighted pair group method using arithmetic averages (UPGMA). The resulting dendrogram grouped the 23 isolates into four main clusters, with genetic distance (Dice coefficient) ranging from 0 to 12%. Almost all isolates from different parts of the same patch were clustered more closely and showed less genetic distance in the UPGMA tree. In the southeast corner of the plot area, a group of patches (#7-#12) in a 2.8 m by 1.2 m area contained ISSR-haplotypes that were also found in other areas of the field (54 m by 17 m). One explanation is that isolates of R. solani spread from this area to the other areas, which is consistent with the mowing pattern that usually starts in this corner. In conclusion, abundant genetic variation among R. solani isolates is present in a small field of creeping bentgrass, but the relationship of isolates as determined by ISSR fingerprinting revealed a population structure that showed evidence of transport between the patches." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zheng, L., F. Shi, and T. Hsiang. 2013. Population structure of a Rhizoctonia solani population in a single field using ISSR markers. Can. J. Plant Pathol. 35(1):p. 131. |
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| DOI: 10.1080/07060661.2013.757422 |
| Web URL(s):
http://www.tandfonline.com/doi/full/10.1080/07060661.2013.757422
Last checked: 07/03/2013
Notes: Item is within a single large file
http://www.tandfonline.com/doi/pdf/10.1080/07060661.2013.757422
Last checked: 07/03/2013
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: b2189612 |
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