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| Web URL(s): | https://turf.rutgers.edu/research/abstracts/symposium2013.pdf#page=41
Last checked: 03/03/2017
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Jespersen, David;
Huang, Bingru |
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| Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
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| Title: | Proteomic profiling for metabolic pathways regulated by cytokinin, ethylene inhibitor and nitrogen for improved heat tolerance in creeping bentgrass |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | New Brunswick, NJ: January 11, 2013 |
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| Source: | Proceedings of the Twenty-Second Annual Rutgers Turfgrass Symposium. 2013, p. 40. |
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| Publishing Information: | New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Abiotic injury; Agrostis stolonifera; Aminoethoxyvinylglycine; Chlorophyll content; Cytokinins; Ethylene inhibitors; Heat stress; Nitrogen application; Urea
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| Cultivar Names: | Penncross |
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| Abstract/Contents: | "Heat is a major abiotic stress affecting creeping bentgrasses leading to premature senescence and decline in overall quality. Previous studies have separately shown that applications of nitrogen, cytokinins or ethylene inhibitors may have a beneficial role for heat tolerance by delaying damaging stress induced senescence, although the mechanisms of such treatments are not fully understood. Penncross plugs were transferred to plastic pots and moved to a growth chamber. Plants were exposed to heat stress at 35/30 C (day/night temperatures) for 4 weeks. Treatments consisted of 1) urea, a commonly used nitrogen source, applied at a rate of 45.36 g / 92.9 meters-2) Zeatin-riboside a naturally occurring form of cytokinin applied at 25 μM concentration, and 3) aminoethoxyvinylglycine, an ethylene inhibitor, applied at 25 μM concentration. Additionally, a combination of all three of the previously mentioned treatments and a control consisting of water were also applied. All were applied at a volume which saturated the canopy three days prior to the stress treatment, and then at weekly intervals during the stress period. Throughout the stress period overall turf quality ratings were taken along with chlorophyll content and membrane stability measurements to estimate the progress of leaf senescence and plant health. At three weeks of heat treatment the ethylene inhibitor, cytokinin and combination treatment all had maintained significantly higher levels of over all quality. The nitrogen and combination treatment had maintained significantly higher chlorophyll content and the ethylene inhibitor, cytokinin, nitrogen and combination treatment all had significantly lower electrolyte leakage compared to the control treatment, demonstrating superior membrane stability also at three weeks of heat stress. Leaf tissue samples were also collected for protein analysis. Proteins were extracted and subjected to 2D SDS-PAGE to separate proteins and software was used to identify those spots that had differentially accumulated during treatment. Proteins altered by the application of nitrogen, cytokinins, and ethylene inhibitor under heat stress included those involved in photosynthesis, energy production and respiration, as well as proteins associated with stress defense, all of which are important metabolic pathways related to heat tolerance. Examining the protein changes associated with these treatments under heat stress will help determine important mechanisms responsible for delaying senescence and this information can than be used to create more heat tolerant plants." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Jespersen, D., and B. Huang. 2013. Proteomic profiling for metabolic pathways regulated by cytokinin, ethylene inhibitor and nitrogen for improved heat tolerance in creeping bentgrass. Proc. Rutgers Turfgrass Symp. p. 40. |
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| Web URL(s):
https://turf.rutgers.edu/research/abstracts/symposium2013.pdf#page=41
Last checked: 03/03/2017
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b3696858 |
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