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| Web URL(s): | https://turf.rutgers.edu/research/abstracts/symposium2013.pdf#page=46
Last checked: 03/03/2017
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Patel, Nrupali;
Oudemans, Peter V.;
Hillman, Bradley I.;
Kobayashi, Donald Y. |
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| Author Affiliation: | Department of Plant Biology & Pathology, Rutgers University |
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| Title: | A viability assay to evaluate direct effects of biological control agents on fungal cells |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | New Brunswick, NJ: January 11, 2013 |
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| Source: | Proceedings of the Twenty-Second Annual Rutgers Turfgrass Symposium. 2013, p. 45. |
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| Publishing Information: | New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Bacteria; Biological control organisms; Disease control; Lysobacter enzymogenes
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| Abstract/Contents: | "There is substantial interest in understanding the mechanisms by which bacteria can biologically control diseases of turfgrass and other plants. However, few methods are available that can quantify direct effects of bacteria on fungal cells. Here we describe an in vitro cell suspension assay using the tetrazolium salt MTT as a viability stain to assess the direct effects of the soilborne bacterium Lysobacter enzymogenes strain C3 on hyphal cells of filamentous fungi. The effects of bacterial cell density, fungal age and the physiological state of fungal mycelia on fungal cell viability were evaluated over 72 h periods. As expected, increased bacterial cell density correlated with reduced fungal cell viability over time. However, bacterial effects on fungal cell viability were also influenced by both age and physiological state of the fungal mycelium. Cells from one-week-old mycelia lost viability at a greater rate compared with two-week-old mycelia. Likewise, loosely-packed hyphal cells obtained from the lower layer of the mycelial pellicle lost viability at a greater rate compared with cells from the hardened upper layer of the mycelial pellicle. Results of this study indicate that the tetrazolium salt MTT can be used effectively to measure reduction in fungal cell viability during interactions with bacteria. Furthermore, this cell suspension assay provides a rapid means to screen antimicrobial agents, including new and novel antifungal compounds as well as potential biological control agents, to evaluate their effectiveness against various turfgrass pathogens." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Patel, N., P. V. Oudemans, B. I. Hillman, and D. Y. Kobayashi. 2013. A viability assay to evaluate direct effects of biological control agents on fungal cells. Proc. Rutgers Turfgrass Symp. p. 45. |
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| Web URL(s):
https://turf.rutgers.edu/research/abstracts/symposium2013.pdf#page=46
Last checked: 03/03/2017
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b3696858 |
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