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| Web URL(s): | https://turf.rutgers.edu/research/abstracts/symposium2013.pdf#page=47
Last checked: 03/03/2017
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Peters, Jeanne S.;
Mohr, Melissa M.;
Gianfagna, Thomas J.;
Meyer, William A. |
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| Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
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| Title: | Detection of Neotyphodium spp. in tall and fine fescue by immunoblot screening |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | New Brunswick, NJ: January 11, 2013 |
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| Source: | Proceedings of the Twenty-Second Annual Rutgers Turfgrass Symposium. 2013, p. 46. |
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| Publishing Information: | New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Acremonium; Endophyte-infected plants; Endophytes; Festuca; Festuca rubra subsp. commutata; Microscopy; Seeds; Tillers (vegetative)
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| Abstract/Contents: | "We have screened tillers and seeds from Festuca spp. plants for the presence of endophyte (Neotyphodium spp.) using an immunoblot kit from Agrinostics, Ltd. Co. (Watkinsville, GA, USA). This kit is a solid phase stacked immunoblot assay in which monoclonal antibodies generated to Neotyphodium spp. cell wall proteins will react to Neotyphodium spp. proteins present in Festuca spp. tillers and seeds. The limit of detection of Neotyphodium spp. in seed is 50 ng/seed and in tillers it is 50 ng/1.6 mm tiller cross section. Immunoblot screening is a more rapid and accurate technique for Neotyphodium detection compared to microscopy. We have screened over 600 plants from a collection of fine fescue from crossing blocks located at the Rutgers Plant Science Research and Extension Farm in Adelphia, NJ. We screened the Chewings fescue collection, the hard fescue collection, and the strong creeping red fescue collection. Eighty-five percent of tillers obtained from the Chewings fescue collection were found to be endophyte positive (E+) and 15% were endophyte negative (E-). Tillers from the hard fescue crossing blocks in Adelphia, NJ were 80% E+ and 20% E-, and 100% of tillers from plants from the strong creeping red fescue crossing blocks were E+. There may be a correlation between presence of endophyte and dollar spot resistance in hard fescue. Seeds were screened from 88 tall fescue cultivars established at the Rutgers Research and Extension Farm in Adelphia, NJ for endophyte as part of the NTEP tall fescue trial. One hundred seeds per cultivar were used for the evaluation. The range of endophyte infection in the tall fescue cultivars ranged from a high of 100% to a low of 0%. There were three cultivars, which had a 0% infection level. There were also eight cultivars, which had infection levels less than 20%. A majority of the cultivars (80%) tested had Neotyphodium infection levels >=90% and the rest (25) had an infection level above 20%, but below 90%. Selections with >=90% infection levels meet the legal requirements for breeding and use at airports attempting to reduce geese and migratory bird populations in take off and landing zones. Endophytes are well-known for the ability to improve environmental and biological stress resistance in grasses, but some produce alkaloids that are detrimental to the health of forage animals. A rapid and accurate method for endophyte screening is critical for selecting plant material that is E+ for turfgrass breeding programs but E- for pasture breeding programs." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Peters, J. S., M. M. Mohr, T. Gianfagna, and W. A. Meyer. 2013. Detection of Neotyphodium spp. in tall and fine fescue by immunoblot screening. Proc. Rutgers Turfgrass Symp. p. 46. |
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| Web URL(s):
https://turf.rutgers.edu/research/abstracts/symposium2013.pdf#page=47
Last checked: 03/03/2017
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: b3696858 |
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