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| Web URL(s): | https://turf.rutgers.edu/research/abstracts/symposium2013.pdf#page=48
Last checked: 03/03/2017
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Roberts, Joseph;
Ma, Bangya;
Tredway, Lane;
Clarke, Bruce;
Ritchie, David |
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| Author Affiliation: | Roberts and Ritchie: Deparment of Plant Pathology, North Carolina State University; Ma: SePRO Corporation; Tredway: Syngenta Crop Protection; Clarke: Department of Plant Biology and Pathology, Rutgers University |
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| Title: | Distribution and pathogenicity of bacteria associated with etiolation or decline in creeping bentgrass |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | New Brunswick, NJ: January 11, 2013 |
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| Source: | Proceedings of the Twenty-Second Annual Rutgers Turfgrass Symposium. 2013, p. 47. |
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| Publishing Information: | New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Acidovorax avenae; Agrostis stolonifera; Microorganisms; Pantoea ananatis; Pseudomonas; Sodium hypochlorite; Xanthomonas; Xanthomonas translucens
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| Abstract/Contents: | "In recent years, bacteria have been implicated as causal agents in multiple cases of decline of creeping bentgrass (CBG; Agrostis stolonifera L.), but little research has been completed to characterize potentially pathogenic bacteria in turf. During 2011 and 2012, 156 bacterial isolates were obtained from CBG turf exhibiting etiolation, chlorosis, or general decline. The majority of the isolates were obtained from the southeastern and mid-Atlantic U.S. Symptomatic tissue was surface sterilized by submersion in 10% sodium hypochlorite for 5 min; disinfested samples were then cut and placed in sterile water allowing the endophytic bacteria to emerge from infected tissue. The resulting suspension was then streaked on nutrient agar and distinct colonies were isolated using the T-streak method. Preliminary identification was obtained by sequencing of the 16S and ITS rDNA regions followed by a BLAST search in GenBank. Sixteen genera including several species of Pseudomonas, Pantoea, Microbacterium, Acidovorax, and Xanthomonas were identified based on high similarity (i.e., >95%) to deposited sequences in GenBank. Selected isolates for each genus were tested for pathogenicity by submerging freshly trimmed 4-wk old 'G-2' CBG seedlings in a bacterial suspension containing 109 CFU ml-1 for 6 min. Inoculated plants were placed on a mist bench (misting 5 min/6 hr) in the greenhouse (28-30 C) for 7-days, followed by placement on a greenhouse bench that received hand-held irrigation applied twice daily. Plants were evaluated periodically for symptom development for 14 days. Acidovorax avenae, Pantoea ananatis, and Xanthomonas translucens visually reduced turf quality compared to the untreated check within 10 days post inoculation. Inoculated plants developed leaf tip dieback that progressed into an overall decline. In the field, the pathogenicity of A. avenae, P. ananatis, and X. translucens was tested by applying 100 ml of a 108 CFU/ml suspension of individual isolates to a 113 cm2 turf area and then covering plants overnight with a clear plastic bowl (355 ml, Amscan Holdings. Inc.). Day and night temperatures during field inoculations were approximately 35 and 20 C, respectively. While dieback or decline was not observed, plants treated with A. avenae had significantly more etiolated tillers 48 hr post inoculation compared to those inoculated with X. translucens, P. ananatis, and non-inoculated plants. Additional research is being conducted to further assess the pathogenicity of X. translucens and P. ananatis on CBG turf." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Roberts, J., B. Ma, L. Tredway, B. Clarke, and D. Ritchie. 2013. Distribution and pathogenicity of bacteria associated with etiolation or decline in creeping bentgrass. Proc. Rutgers Turfgrass Symp. p. 47. |
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| Web URL(s):
https://turf.rutgers.edu/research/abstracts/symposium2013.pdf#page=48
Last checked: 03/03/2017
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: b3696858 |
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