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DOI: | 10.3852/08-065 |
Web URL(s): | http://www.mycologia.org/content/100/6/867.full Last checked: 07/05/2013 Access conditions: Item is within a limited-access website http://www.mycologia.org/content/100/6/867.full.pdf Last checked: 07/05/2013 Requires: PDF Reader Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Lakshman, Dilip K.;
Natarajan, Savithiry S.;
Lakshman, Sukla;
Garrett, Wesley M.;
Dhar, Arun K. |
Author Affiliation: | Lakshman, D. K.: Floral and Nursery Plants Research Unit, USDA-ARS, Beltsville, Maryland; Natarajan and Lakshman, S.: USDA-ARS Soybean Genomics and Improvement Laboratory, BARC West, Beltsville, Maryland; Garrett: Animal Biosciences and Biotechnology Laboratory, Beltsville, Maryland; Dhar: Advanced Bionutrition Corp., Columbia, Maryland |
Title: | Optimized protein extraction methods for proteomic analysis of Rhizoctonia solani |
Section: | Genetics/molecular biology Other records with the "Genetics/molecular biology" Section
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Source: | Mycologia. Vol. 100, No. 6, November/December 2008, p. 867-875. |
Publishing Information: | Lancaster, Pennsylvania: New Era Print Co. for the New York Botanical Garden |
# of Pages: | 9 |
Related Web URL: | http://www.mycologia.org/content/100/6/867.abstract Last checked: 07/05/2013 Notes: Abstract only |
Keywords: | TIC Keywords: Analytical methods; Basidiomycota; Biological control organisms; Comparisons; Densitometer; Fungus profile; Host pathogen interaction system; Mass spectrometry; Mycology; Organic matter; Peptides; Protein extraction; Rhizoctonia solani; Root diseases; Saprophyte; Thanatephorus cucumeris
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Abstract/Contents: | "Rhizoctonia solani (Teleomorph: Thanatephorus cucumeris, T. praticola) is a basidiomycetous fungus and a major cause of root diseases of economically important plants. Various isolates of this fungus are also beneficially associated with orchids, may serve as biocontrol agents or remain as saprophytes with roles in decaying and recycling of soil organic matter. R. solani displays several hyphal anastomosis groups (AG) with distinct host and pathogenic specializations. Even though there are reports on the physiological and histological basis of Rhizoctonia-host interactions, very little is known about the molecular biology and control of gene expression early during infection by this pathogen. Proteomic technologies are powerful tools for examining alterations in protein profiles. To aid studies on its biology and host pathogen interactions, a twodimensional (2-D) gel-based global proteomic study has been initiated. To develop an optimized protein extraction protocol for R. solani, we compared two previously reported protein extraction protocols for 2-D gel analysis of R. solani (AG-4) isolate Rs23. Both TCA-acetone precipitation and phosphate solubilization before TCA-acetone precipitation worked well for R. solani protein extraction, although selective enrichment of some proteins was noted with either method. About 450 spots could be detected with the densitiometric tracing of Coomassie blue-stained 2-D PAGE gels covering pH 47 and 6.5205 kDa. Selected protein spots were subjected to mass spectrometric analysis with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). Eleven protein spots were positively identified based on peptide mass fingerprinting match with fungal proteins in public databases with the Mascot search engine. These results testify to the suitability of the two optimized protein extraction protocols for 2-D proteomic studies of R. solani." |
Language: | English |
References: | 55 |
Note: | Gel images Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Lakshman, D. K., S. S. Natarajan, S. Lakshman, W. M. Garrett, and A. K. Dhar. 2008. Optimized protein extraction methods for proteomic analysis of Rhizoctonia solani. Mycologia. 100(6):p. 867-875. |
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| DOI: 10.3852/08-065 |
| Web URL(s): http://www.mycologia.org/content/100/6/867.full Last checked: 07/05/2013 Access conditions: Item is within a limited-access website http://www.mycologia.org/content/100/6/867.full.pdf Last checked: 07/05/2013 Requires: PDF Reader Access conditions: Item is within a limited-access website |
| MSU catalog number: b2214983 MSU catalog number: b5343430 |
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