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DOI: | 10.3852/11-365 |
Web URL(s): | http://www.mycologia.org/content/104/5/1250.full Last checked: 09/18/2015 Access conditions: Item is within a limited-access website http://www.mycologia.org/content/104/5/1250.full.pdf Last checked: 09/18/2015 Requires: PDF Reader Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Zhao, Shuang;
Clarke, Bruce B.;
Shen, Qirong;
Zhang, Lisa;
Zhang, Ning |
Author Affiliation: | Zhao and Shen: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, China; Zhao, Clarke and Zhang, N.: Plant Biology and Pathology, Rutgers University, New Brunswick; Zhang, L.: East Brunswick High School, East Brunswick, New Jersey |
Title: | Development and application of a TaqMan real-time PCR assay for rapid detection of Magnaporthe poae |
Section: | Techniques Other records with the "Techniques" Section
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Source: | Mycologia. Vol. 104, No. 5, September/October 2012, p. 1250-1259. |
Publishing Information: | Lancaster, Pennsylvania: New Era Print Co. for the New York Botanical Garden |
# of Pages: | 10 |
Related Web URL: | http://www.mycologia.org/content/104/5/1250.abstract Last checked: 07/08/2013 Notes: Abstract only |
Keywords: | TIC Keywords: Disease identification; Festuca; Magnaporthe poae; Poa; Poa pratensis; Polymerase chain reaction
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Abstract/Contents: | "In North America, one of the most important root diseases of Poa and Festuca turf is summer patch, caused by Magnaporthe poae. Detection and identification of M. poae in infected roots by conventional culture-based methods is difficult and time consuming, typically taking 3 wk or longer to accomplish. In this study, a culture-independent, TaqMan real-time PCR assay was developed for the detection of M. poae from the roots of fungicide treated and non-treated Kentucky bluegrass (Poa pratensis) turf. The assay was validated with the target pathogen, closely related fungal species and a number of other microorganisms that inhabit the same host and soil environment. This assay was more sensitive (could detect as little as 3.88 pg genomic DNA of M. poae), rapid and accurate compared to direct microscopic observation and isolation on a selective medium. The real-time PCR detection results corresponded closely to visual assessments of disease severity in the field. Utilization of this assay in diagnostic laboratories will enable turfgrass managers to more quickly and effectively detect and potentially reduce fungicide usage through early and accurate identification of the pathogen." |
Language: | English |
References: | 42 |
Note: | Pictures, b/w Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Zhao, S., B. B. Clarke, Q. Shen, L. Zhang, and N. Zhang. 2012. Development and application of a TaqMan real-time PCR assay for rapid detection of Magnaporthe poae. Mycologia. 104(5):p. 1250-1259. |
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| DOI: 10.3852/11-365 |
| Web URL(s): http://www.mycologia.org/content/104/5/1250.full Last checked: 09/18/2015 Access conditions: Item is within a limited-access website http://www.mycologia.org/content/104/5/1250.full.pdf Last checked: 09/18/2015 Requires: PDF Reader Access conditions: Item is within a limited-access website |
| MSU catalog number: b2214983 MSU catalog number: b5343430 |
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